Castration induces apoptosis in the ventral prostate but not in an androgen-sensitive prostatic adenocarcinoma in the rat.
Castration induces apoptosis in the ventral prostate but not in an androgen-sensitive prostatic adenocarcinoma in the rat.
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去势会诱导大鼠腹侧前列腺细胞凋亡，但不会诱导雄激素敏感的前列腺腺癌细胞凋亡。
作者：
Anna Brandstrom;P. Westin;A. Bergh;S. Cajander;J. Damber
研究雄激素敏感的Dunning R3327 PAP前列腺腺癌去势后14天的细胞凋亡情况，并与腹侧前列腺进行比较。应用Northern blot和免疫组化方法分析了睾丸激素抑制前列腺信息2和组织型纤溶酶原激活物在Dunning肿瘤和前列腺腹侧组织中的mRNA表达。用凝胶电泳法检测基因组DNA内切酶降解程度。用原位末端标记法鉴定肿瘤上皮细胞凋亡。去势后的前列腺腹侧和Dunning肿瘤中含有溴脱氧尿苷（BrdUrd）的上皮细胞通过免疫染色定位。雄激素消融导致正常前列腺中睾酮抑制的前列腺信息-2和组织型纤溶酶原激活物转录的诱导，并在去势后约2至5天达到峰值。Dunning前列腺肿瘤中的这些转录物水平在同一时期没有表现出任何诱导作用。硫酸糖蛋白-2和组织型纤溶酶原激活剂的免疫组化染色证实了肿瘤组织和前列腺腹侧在转录水平上的差异。DNA完整性的测定也显示出类似的结果，肿瘤中DNA的碎片化程度远低于前列腺腹侧DNA的初始和显著降解。去势没有增加原位末端标记上皮肿瘤细胞的数量。BrdUrd免疫检测显示，去势诱导前列腺腹侧BrdUrd阳性上皮细胞数量的初始增加。在肿瘤中，去势导致brdurd阳性上皮细胞减少。我们得出结论，在雄激素敏感型前列腺Dunning R3327 PAP腺癌中，导致细胞凋亡的生化级联反应不像腹侧前列腺那样被雄激素停药激活。
Apoptosis in the androgen-sensitive Dunning R3327 PAP prostatic adenocarcinoma was studied during the post castration period of 14 days and compared with the ventral prostate. The mRNA expression of testosterone repressed prostatic message-2 and tissue-type plasminogen activator in the Dunning tumor and in the ventral prostate was analyzed by Northern blot experiments and immunohistochemical procedures. The degree of endonuclease-degraded genomic DNA was examined by gel electrophoresis. Apoptotic tumor epithelial cells were identified with in situ end labeling. Epithelial cells incorporating bromodeoxyuridine (BrdUrd) after castration in the ventral prostate and the Dunning tumors were localized with immunostaining. Androgen ablation resulted in an induction of testosterone repressed prostatic message-2 and tissue-type plasminogen activator transcripts in the normal prostate with a peak at approximately 2 to 5 days post castration. These transcript levels in the Dunning prostatic tumors did not show any induction during the same period. Immunohistochemical staining for sulfated glycoprotein-2 and tissue-type plasminogen activator confirmed this difference between the tumor tissue and the ventral prostate at the transcriptional level. The determination of DNA integrity showed similar results in that the degree of DNA fragmentation in the tumor was much lower than the initial and marked degradation of DNA in the ventral prostate. The number of in situ end-labeled epithelial tumor cells were not increased by castration. BrdUrd immunodetection showed that castration induced an initial increase in the number of BrdUrd-positive epithelial cells in the ventral prostate. In the tumors, castration resulted in a decrease in BrdUrd-positive epithelial cells. It was concluded that in the androgen-sensitive prostatic Dunning R3327 PAP adenocarcinoma, the biochemical cascade leading to apoptosis is not activated by androgen withdrawal, as in the ventral prostate.
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非增殖性雄激素依赖性前列腺癌细胞的程序性死亡。
影响因子：
11.2
作者：
Martikainen,P;Kyprianou,N;Tucker,RW;Isaacs,JT
通讯作者：
Isaacs,JT
雄激素消融后 PC-82 人类前列腺癌消退过程中的程序性细胞死亡。
影响因子：
11.2
作者：
Kyprianou,N;English,HF;Isaacs,JT
通讯作者：
Isaacs,JT
大鼠体内肿瘤大小与化疗激素联合治疗前列腺癌治愈率之间的关系。
影响因子：
11.2
作者：
Isaacs,JT
通讯作者：
Isaacs,JT
DOI：
10.1016/b978-0-12-571139-5.50018-5
发表时间：
1983
期刊：
Recent progress in hormone research
影响因子：
--
作者：
Gerald R. Cunha;L. W. Chung;John M. Shannon;O. Taguchi;H. Fujii
通讯作者：
Gerald R. Cunha;L. W. Chung;John M. Shannon;O. Taguchi;H. Fujii
影响因子：
11.2
作者：
M. Gleave;J. Hsieh;H. Wu;A. C. Eschenbach;Leland W.K. Chung
通讯作者：
M. Gleave;J. Hsieh;H. Wu;A. C. Eschenbach;Leland W.K. Chung