THE EXPRESSION OF SUBUNIT-C CORRELATES WITH AND THUS MAY LIMIT THE BIOSYNTHESIS OF THE MITOCHONDRIAL F0F1-ATPASE IN BROWN ADIPOSE-TISSUE
THE EXPRESSION OF SUBUNIT-C CORRELATES WITH AND THUS MAY LIMIT THE BIOSYNTHESIS OF THE MITOCHONDRIAL F0F1-ATPASE IN BROWN ADIPOSE-TISSUE
复制标题
DOI：
10.1074/jbc.270.13.7689
复制
发表时间：
1995-03-31
影响因子：
4.8
通讯作者：
CANNON, B
中科院分区：
文献类型：
--
作者：
HOUSTEK, J;ANDERSSON, U;CANNON, B
先前发现，棕色脂肪组织(BAT)中线粒体ATPase的低含量与ATPase F-1部分的β亚基的高水平转录和线粒体编码亚基的高水平转录形成鲜明对比(Houstek，J.，Tvrdik，P.，Pavelka，S.和Baudysova，M.(1991)Fees Lett)。294、191-194)。为了确定哪个亚基限制了ATPase复合体的合成，我们研究了编码ATPase催化F-1部分的α、β和伽马亚基以及F-0部分的b、c、d和OSCP亚基的核基因的表达。与小鼠的其他组织相比，在BAT中发现了高水平的α-F-1、β-F-1、伽马-F-1、b-F-0、d-F-0和OSCP的转录。在BAT中低水平表达的基因只有c-F-0亚基基因。BAT组织中c-F_1的转录水平比其他组织低4-70倍。在成年大鼠和金黄地鼠的BAT和新生羔羊的BAT中发现了类似的ATPase基因的表达模式，与其他哺乳动物相比，线粒体ATPase的含量较高，相应地，c-F-0基因的表达水平也较高。c-F-0基因的表达与仓鼠EAT的个体发育也有很好的相关性，在出生后第一周线粒体不产热且ATPase含量相对较高的时候，c-F-0基因的表达较高，但在随后的几天，当ATPase含量下降时，表达水平较低。随着产热功能的发展，c-P-0基因的表达和随后膜固有的F-0部分疏水亚基c的合成可能控制着蝙蝠ATPase复合体的生物合成。在其他组织中，c-F-0亚单位也可以发挥类似的调节作用。
A low content of mitochondrial ATPase in brown adipose tissue (BAT) has previously been found to contrast with high levels of the transcripts of the beta-subunit of the F-1 part of the ATPase and of the transcripts of the mitochondrial encoded subunits (Houstek, J., Tvrdik, P., Pavelka, S., and Baudysova, M. (1991) FEES Lett. 294, 191-194). To delineate which subunit limits the synthesis of the ATPase complex, we have studied the expression of the nuclear genes encoding subunits alpha, beta, and gamma of the catalytic F-1 part and the b, c, d, and OSCP subunits of the F-0 part of the ATPase.In comparison with other tissues of mice, high levels of transcripts of alpha-F-1, beta-F-1, gamma-F-1, b-F-0, d-F-0, and OSCP were found in BAT. The only genes expressed at a low level in BAT were those of the c-F-0 subunit. The levels of c-F, transcripts were 4-70-fold lower in BAT than in other tissues. An analogous expression pattern of the ATPase genes was found in BAT of adult rat and hamster, In BAT of newborn lamb, which, in contrast to other mammals, has a high content of mitochondrial ATPase, correspondingly high levels of c-F-0 mRNA were found, Expression of the c-F-0 genes also correlated well with the ontogenic development of EAT in the hamster, being high during the first postnatal week when mitochondria are nonthermogenic and contain a relatively high amount of ATPase, but low on subsequent days when ATPase content decreases, as the thermogenic function develops.It is suggested that expression of the c-P-0 genes and subsequent synthesis of the hydrophobic subunit c of the membrane-intrinsic F-0 part of the enzyme may control the biosynthesis of the ATPase complex in BAT. An analogous regulatory role of the c-F-0 subunit could be postulated in other tissues.