Proteome profiling for assessing diversity: analysis of individual heads of Drosophila melanogaster using LC-ion mobility-MS.
Proteome profiling for assessing diversity: analysis of individual heads of Drosophila melanogaster using LC-ion mobility-MS.
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DOI：
10.1021/pr050037o
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发表时间：
2005-06
影响因子：
4.4
通讯作者：
J. Taraszka;Xinfeng Gao;S. Valentine;Renã A. Sowell;Stormy L Koeniger;D. F. Miller;T. Kaufman;D. Clemmer
中科院分区：
文献类型：
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作者：
J. Taraszka;Xinfeng Gao;S. Valentine;Renã A. Sowell;Stormy L Koeniger;D. F. Miller;T. Kaufman;D. Clemmer
已分析和比较的蛋白质组的三个头的个体果蝇生物体的液相色谱法，离子迁移谱，质谱法的方法相结合。在所有三个个体中总共鉴定了197种蛋白质（平均每个个体120 +/-20种蛋白质），其中至少101种蛋白质存在于所有三个个体中。在所有三个数据集中，观察到超过25000个分子离子（平均每个个体9000 +/- 2000）对应于单个肽的质子化前体离子。数据集之间的峰的比较显示，与在所有头部中发现的质子化肽相对应的峰比出现在成对个体之间或仅在一个个体内的那些特征更强烈。此外，在所有个体中共有的峰的相对强度几乎没有变化。似乎是蛋白质组中丰度较低的组分在确定个体的独特特征方面发挥着最重要的作用。
The proteomes of three heads of individual Drosophila melanogaster organisms have been analyzed and compared by a combination of liquid chromatography, ion mobility spectrometry, and mass spectrometry approaches. In total, 197 proteins are identified among all three individuals (an average of 120 +/- 20 proteins per individual), of which at least 101 proteins are present in all three individuals. Within all three datasets, more than 25 000 molecular ions (an average of 9000 +/- 2000 per individual) corresponding to protonated precursor ions of individual peptides have been observed. A comparison of peaks among the datasets reveals that peaks corresponding to protonated peptides that are found in all heads are more intense than those features that appear between pairs of or within only one of the individuals. Moreover, there is little variability in the relative intensities of the peaks common among all individuals. It appears that it is the lower abundance components of the proteome that play the most significant role in determining unique features of individuals.