An electrochemical study of microporous track-etched membrane permeability and the effect of surface protein layers
An electrochemical study of microporous track-etched membrane permeability and the effect of surface protein layers
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DOI：
10.1016/j.colsurfb.2017.06.032
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发表时间：
2017-10-01
影响因子：
5.8
通讯作者：
Vadgama, P.
中科院分区：
文献类型：
--
作者：
Adatia, K.;Raja, M.;Vadgama, P.
微孔径迹蚀刻膜作为重要的渗透性生长表面的细胞培养，扩散溶质运输需要跨越两个生长室。本研究建立了有效的溶质扩散系数为四个探针微溶质：过氧化氢，邻苯二酚，对乙酰氨基酚和抗坏血酸在三个轨道蚀刻膜制定，分别从聚碳酸酯和聚对苯二甲酸乙二醇酯。计时电流法和循环伏安法用于扩散测量。这些显示相对于可用孔面积，孔内扩散显著降低。扩散系数范围为1.43 x 10（-10）至3.17 x 10（-7）cm（2）s（-1）。这有力地表明，水组织在微孔是不等同于散装水。I型和IV型胶原蛋白、I型胶原蛋白-纤连蛋白、I型胶原蛋白-肝素和I型胶原蛋白-硫酸软骨素的叠加蛋白层增加了扩散阻力，但由于胶原蛋白-肝素和胶原蛋白-硫酸软骨素组合的电荷排斥，抗坏血酸盐扩散具有不成比例的阻滞。天然肌腱和软骨的扩散阻力要小得多;扩散系数范围为8.33 x 10（-6）至1.09 x 10（-8）cm（2）s（-1）。(C)2017爱思唯尔B. V.保留所有权利。
Microporous track-etched membranes serve as important permeable growth surfaces for cell culture where diffusive solute transport is needed across two growth compartments. This study has established effective solute diffusion coefficients for four probe micro-solutes: hydrogen peroxide, pyrocatechol, acetaminophen and ascorbic acid across three track-etched membranes formulated, respectively, from polycarbonate and polyethylene terephthalate. Chronoamperometry and cyclic voltammetry were used for the diffusion measurements. These showed substantially reduced intra-pore diffusion in relation to available pore area. Diffusion coefficients ranging from 1.43 x 10(-10) to 3.17 x 10(-7) cm(2) s(-1) were demonstrated. This strongly suggests that water organisation in micro-pores is not equivalent to that of bulk water. Superimposed protein layers of Type I and IV collagen, Type I collagen-fibronectin, Type I collagen-heparin, and Type I collagen-chondroitin sulphate increased diffusional resistance, but with disproportional retardation of ascorbate diffusion due to charge repulsion at collagen-heparin and collagen-chondroitin sulphate combinations. Diffusive resistance at natural tendon and cartilage was considerably smaller; diffusion coefficients ranged from 8.33 x 10(-6) to 1.09 x 10(-8) cm(2) s(-1). (C) 2017 Elsevier B.V. All rights reserved.