Identification and characterization of the Bcl-2- associated athanogene (BAG) protein family in rice
Identification and characterization of the Bcl-2- associated athanogene (BAG) protein family in rice
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DOI：
10.5897/ajb11.3474
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发表时间：
2011-01
影响因子：
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通讯作者：
R. Rana;S. Dong;Z. Ali;A. Khan;Hongsheng Zhang
中科院分区：
文献类型：
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作者：
R. Rana;S. Dong;Z. Ali;A. Khan;Hongsheng Zhang
Bcl-2-associated athanogene（BAG）蛋白参与动物Hsp 70/HSC 70的调节。人类有6个BAG基因，编码9种不同亚细胞定位的亚型。据报道，拟南芥含有7个BAG蛋白。我们使用profile-sequence（Pfam）和profile-profile（FFAS）算法搜索了水稻BAG蛋白，发现了6个同源物。BAG蛋白家族在O.根据其它保守结构域的存在，可以将sativa分为两类。第一类由四个OsBAG基因（1至4）含有额外的泛素样结构域，结构上类似于人类BAG 1蛋白，可能是植物中的BAG 1直系同源物。II类由两个OsBAG基因（5和6）组成，含有钙调蛋白结合结构域。O.水稻BAG蛋白具有保守的表面电荷（OsBAG 5除外）和BAG结构域与Hsp 70核苷酸结合结构域（NB）结合的关键残基。Meta分析表明，OsBAG基因在不同胁迫（生物胁迫和非生物胁迫）下表达上调或下调。热胁迫下OsBAG基因的实时荧光定量PCR结果表明，热胁迫1 h后，OsBAG基因的表达量均达到最大值，随后表达量逐渐下降，到24 h时恢复到基础水平。这些结果表明OsBAG基因可能在热胁迫的发生中起重要作用。对该基因家族成员的深入研究将有助于进一步了解植物细胞程序性死亡（PCD）机制。关键词：水稻，类泛素结构域，核苷酸结合结构域，实时荧光定量PCR。
The Bcl-2-associated athanogene (BAG) proteins are involved in the regulation of Hsp70/HSC70 in animals. There are six BAG genes in human that encode nine isoforms with different subcellular locations. Arabidopsis thaliana is reported to contain seven BAG proteins. We searched BAG proteins in Oryza sativa using profile-sequence (Pfam) and profile-profile (FFAS) algorithms and found six homologs. The BAG protein family in O. sativa can be grouped into two classes based on the presence of other conserved domains. Class I consists of four OsBAG genes (1 to 4) containing an additional ubiquitin-like domain, structurally similar to the human BAG1 proteins and might be BAG1 orthologs in plants. Class II consists of two OsBAG genes (5 and 6) containing calmodulin-binding domain. Multiple sequence alignment and structural models of O. sativa BAG proteins showed conservation of surface charge (except OsBAG5) and critical residues for the binding of BAG domain to Hsp70 nucleotide binding domain (NB). Meta analysis of microarray data showed that OsBAG genes are up or down regulated under different stresses (biotic and abiotic). Data obtained from real-time PCR of OsBAG genes under heat stress showed that maximum induction in the expression of all the genes occurred after one hour exposure to heat stress, while reduction in the expression was observed in the following time course and ultimately returned to the basal level at 24 h treatment. These results suggest that OsBAG genes might play important role at the onset of heat stress. A further detailed study may explore the exact function of the members of this gene family and help to make understanding of programmed cell death (PCD) mechanism in plants. Key words : Rice, ubiquitin-like domain, nucleotide-binding domain, real-time PCR.