Double strand RNA delivery system for plant-sap-feeding insects.
Double strand RNA delivery system for plant-sap-feeding insects.
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DOI：
10.1371/journal.pone.0171861
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Gundersen-Rindal DE
中科院分区：
文献类型：
--
作者：
Ghosh SK;Hunter WB;Park AL;Gundersen-Rindal DE
双链 RNA (dsRNA) 介导的基因沉默，也称为 RNA 干扰 (RNAi)，是功能基因组研究的一项突破性技术，也是防治害虫的潜在工具。自从 RNAi 出现以来，大量研究记录了将外源合成的 dsRNA 或 siRNA 成功引入生物体，通过降解与所提供的 siRNA 同源的内源 RNA，触发高效的基因沉默。管理半翅目害虫，尤其是褐纹臭蝽 (BMSB)，对于粮食生产力至关重要。 BMSB 最近被引入北美，它既是高价值特种作物、行耕作物和主粮作物的入侵性农业害虫，也是室内有害害虫。 RNAi 技术可能是控制这种贪婪害虫的可行工具，但将 dsRNA 传递给刺吸式昆虫却带来了巨大的挑战。有效且实际地使用 RNAi 作为分子生物农药来生物防治环境中的 BMSB 等昆虫，需要通过摄入在体内递送 dsRNA。因此，分子生物学家在开发昆虫特异性分子生物农药时面临的关键挑战是找到有效且可靠的方法来实际递送稳定的双链RNA，例如通过口服摄入。这里展示了一种可靠的有效昆虫特异性 dsRNA 递送系统，通过新的递送系统口服喂养，诱导 JHAMT 和 Vg 等目标基因表达显着降低。这种最先进的递送方法克服了环境递送挑战，从而通过口服递送给半翅目和其他害虫的昆虫特异性 dsRNA 来诱导 RNAi。
Double-stranded RNA (dsRNA)-mediated gene silencing, also known as RNA interference (RNAi), has been a breakthrough technology for functional genomic studies and represents a potential tool for the management of insect pests. Since the inception of RNAi numerous studies documented successful introduction of exogenously synthesized dsRNA or siRNA into an organism triggering highly efficient gene silencing through the degradation of endogenous RNA homologous to the presented siRNA. Managing hemipteran insect pests, especially Halyomorpha halys (Stål) (Heteroptera: Pentatomidae), the brown marmorated stink bug (BMSB), is critical to food productivity. BMSB was recently introduced into North America where it is both an invasive agricultural pest of high value specialty, row, and staple crops, as well as an indoor nuisance pest. RNAi technology may serve as a viable tool to manage this voracious pest, but delivery of dsRNA to piercing-sucking insects has posed a tremendous challenge. Effective and practical use of RNAi as molecular biopesticides for biocontrol of insects like BMSB in the environment requires that dsRNAs be delivered in vivo through ingestion. Therefore, the key challenge for molecular biologists in developing insect-specific molecular biopesticides is to find effective and reliable methods for practical delivery of stable dsRNAs such as through oral ingestion. Here demonstrated is a reliable delivery system of effective insect-specific dsRNAs through oral feeding through a new delivery system to induce a significant decrease in expression of targeted genes such as JHAMT and Vg. This state-of-the-art delivery method overcomes environmental delivery challenges so that RNAi is induced through insect-specific dsRNAs orally delivered to hemipteran and other insect pests.