High expression of PFTK1 in cancer cells predicts poor prognosis in colorectal cancer
High expression of PFTK1 in cancer cells predicts poor prognosis in colorectal cancer
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DOI：
10.3892/mmr.2017.6560
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发表时间：
2017-07-01
影响因子：
3.4
通讯作者：
Li, Xiaoqiang
中科院分区：
文献类型：
--
作者：
Mao, Youjun;Jia, Yuqing;Li, Xiaoqiang
丝氨酸/苏氨酸蛋白激酶PFTAIRE 1（PFTK 1）是细胞周期蛋白依赖性激酶家族的成员，其在几种恶性肿瘤中高度表达，包括肝细胞癌、食管癌、乳腺癌和胃癌以及神经胶质瘤。它有助于肿瘤进展并影响肿瘤预后。然而，PFTK 1在人结直肠癌（CRC）中的表达及其临床病理意义仍有待阐明。本研究旨在检测PFTK 1在人结直肠癌中的表达，并评价其表达的临床意义。采用逆转录-定量聚合酶链反应（RT-PCR）方法检测10例新鲜结直肠癌组织和10例癌旁正常组织中PFTK 1 mRNA的表达，并进行比较。对179例结直肠癌组织标本和47例正常结直肠病变对照标本进行免疫组化，以表征PFTK 1蛋白的表达。采用Kaplan-Meier生存率（OS）和考克斯回归分析评价结直肠癌患者的预后。PFTK 1 mRNA在CRC组织中的表达（1.433 +/- 0.168）显著高于正常组织（0.853 +/- 0.107; t=1.97（“t”是根据配对t检验从mRNA数据定量获得的值），P=0.008）。PFTK 1在92例（51.40%）结直肠癌组织中呈高表达，其表达与结直肠癌的TNM分期（P=0.042）、肿瘤分级（P =0.022）及术前癌胚抗原（CEA）水平（P
The serine/threonine-protein kinase PFTAIRE 1 (PFTK1) is a member of the cyclin-dependent kinase family that is highly expressed in several malignant tumors, including hepatocellular carcinoma, esophageal, breast and gastric cancers, and glioma. It contributes to tumor progression and influences tumor prognosis. However, the expression and clinicopathological significance of PFTK1 in human colorectal cancer (CRC) remain to be elucidated. The present study aimed to examine the expression of PFTK1 and to evaluate the clinical significance of its expression in human CRC. Reverse transcription-quantitative polymerase chain reaction was performed on 10 fresh CRC and 10 surrounding normal tissue samples to detect and compare the expression of PFTK1 mRNA in CRC and normal colorectal tissues. Immunohistochemistry was performed on 179 CRC tissue specimens and 47 control samples of normal colorectal lesions to characterize the expression of PFTK1 protein. Kaplan-Meier overall survival (OS) rate and Cox regression analyses were performed to evaluate the prognosis of patients with CRC. The expression of PFTK1 mRNA in CRC tissues (1.433 +/- 0.168) was significantly higher compared with normal tissues (0.853 +/- 0.107; t=1.97 ('t' was the value obtained from quantification of the mRNA data, following a paired t-test), P=0.008). High PFTK1 expression in cancerous cells was detected in 92 of the CRC specimens (51.40%), and high levels of PFTK1 were associated with tumor node metastasis (TNM) stage (P=0.042), tumor classification (P=0.022) and preoperative carcinoembryonic antigen (CEA) level (P