METHOD FOR THE DETERMINATION OF OXYGEN-CONSUMPTION RATES AND DIFFUSION-COEFFICIENTS IN MULTICELLULAR SPHEROIDS
METHOD FOR THE DETERMINATION OF OXYGEN-CONSUMPTION RATES AND DIFFUSION-COEFFICIENTS IN MULTICELLULAR SPHEROIDS
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DOI：
10.1016/s0006-3495(84)84030-8
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发表时间：
1984-01-01
影响因子：
3.4
通讯作者：
MUELLERKLIESER, W
中科院分区：
文献类型：
--
作者：
MUELLERKLIESER, W
建立了一种用氧敏感微电极测量多细胞球体中氧分压(PO2)分布的方法。在搅拌的生长介质中，多细胞肿瘤球体[EMT6/Ro细胞]的特征是在球体周围有扩散耗竭的区域。这一区域是由靠近球体的一层未搅拌的介质引起的，导致PO2值从体相介质向球体表面不断下降。理论研究表明，可以通过测量球体外扩散耗尽区中的PO2梯度来评估球体中与体积有关的氧耗率Q。因此，可以通过测量球体内的PO2梯度来确定球体内的Krogh‘’S扩散常数KS。多细胞球体是一种有用的体外肿瘤模型，可用于实验和理论分析肿瘤细胞供氧、肿瘤组织中的氧代谢和癌细胞对治疗的反应之间的相互关系。
A method was developed for the quantitative evaluation of oxygen tension (PO2) distributions in multicellular spheroids measured with O2-sensitive microelectrodes. Multicellular tumor spheroids [EMT6/Ro cells] in stirred growth media were characterized by a diffusion-depleted zone surrounding the spheroids. This zone was elicited by an unstirred layer of medium next to the spheroid leading to a continuous decrease in the PO2 values from the bulk medium towards the spheroid surface. Theoretical considerations demonstrate that the volume-related O2 consumption rate, Q, in the spheroids can be assessed by measuring the PO2 gradient in the diffusion-depleted zone outside the spheroids. Accordingly, Krogh''s diffusion constant, KS, in the spheroids can be determined through measuring the PO2 gradient within the spheroids. Multicellular spheroids represent useful in vitro tumor models for the experimental and theoretical analysis of the interrelationship among O2 supply to tumor cells, O2 metabolism in tumor tissue and responsiveness of cancer cells to treatment.