AMINERGIC MODULATION IN LOBSTER STOMATOGASTRIC GANGLION .2. TARGET NEURONS OF DOPAMINE, OCTOPAMINE, AND SEROTONIN WITHIN THE PYLORIC CIRCUIT
AMINERGIC MODULATION IN LOBSTER STOMATOGASTRIC GANGLION .2. TARGET NEURONS OF DOPAMINE, OCTOPAMINE, AND SEROTONIN WITHIN THE PYLORIC CIRCUIT
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DOI：
10.1152/jn.1986.55.5.866
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发表时间：
1986-05-01
影响因子：
2.5
通讯作者：
HARRISWARRICK, RM
中科院分区：
文献类型：
--
作者：
FLAMM, RE;HARRISWARRICK, RM
在前面的论文中，我们描述了多巴胺，章鱼胺，和5-羟色胺调节神经回路产生一个良好的描述运动模式，幽门节律的口胃神经节的多刺龙虾，龙虾中断。在本文中，我们确定的神经元内的幽门电路，直接受到每种胺。我们通过将每个幽门神经元从所有已知的突触输入中分离出来，使用突触前神经元的荧光黄光灭活和幽门神经递质的药理学阻断的组合来实现这一点。将多巴胺、章鱼胺和5-羟色胺应用于制备物，并记录突触分离的神经元的反应。每种胺对幽门回路的神经元都有独特的影响。回路中的几乎每一个神经元都直接受到每一种胺的影响。多巴胺和章鱼胺调节每个神经元，而血清素影响六种细胞类型中的四种。每种胺在幽门神经元中具有多种作用，包括诱导内源性节律性爆发活动，启动或增强强直性放电活动，以及伴随超极化的抑制。所有三种胺诱导一个神经元（AB神经元）的节律性爆发，但与多巴胺和5-羟色胺相比，章鱼胺引起的慢波膜电位振荡的形式不同。我们对每种胺对每个幽门神经元的影响的了解，结合对幽门回路突触组织的广泛了解，使我们能够定性地解释每种胺在突触完整回路中产生的幽门运动节律的独特变体的主要方面。
In the preceding paper, we describe how dopamine, octopamine, and serotonin modulate the neural circuit generating a well-described motor pattern, the pyloric rhythm of the stomatogastric ganglion in the spiny lobster, Panulirus interruptus. In this paper, we identify the neurons within the pyloric circuit that are directly affected by each amine. We accomplished this by isolating each pyloric neuron from all known synaptic input, using a combination of Lucifer yellow photoinactivation of presynaptic neurons and pharmacological blockade by pyloric neurotransmitters. Dopamine, octopamine, and serotonin were bath applied to the preparation, and the responses of synaptically isolated neurons were recorded. Each amine had a unique constellation of effects on the neurons of the pyloric circuit. Almost every neuron in the circuit was directly affected by each amine. Dopamine and octopamine modulated every neuron, whereas serotonin affected four of the six cell types. Each amine had multiple effects among pyloric neurons including the induction of endogenous rhythmic bursting activity, initiation or enhancement of tonic firing activity, and inhibition accompanied by hyperpolarization. All three amines induced rhythmic bursting in one neuron (the AB neuron), but the form of the underlying slow-wave membrane-potential oscillations was different with octopamine than with dopamine and serotonin. Our knowledge of the effects of each amine on each pyloric neuron, combined with the extensive knowledge of the synaptic organization of the pyloric circuit, has allowed us to explain qualitatively the major aspects of the unique variants of the pyloric motor rhythm that each amine produces in the synaptically intact circuit.