Biodegradable thermosensitive injectable PEG-PCL-PEG hydrogel for bFGF antigen delivery to improve humoral immunity
Biodegradable thermosensitive injectable PEG-PCL-PEG hydrogel for bFGF antigen delivery to improve humoral immunity
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DOI：
10.3109/08977190903159938
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发表时间：
2009-12-01
期刊：
影响因子：
1.8
通讯作者：
Qian, Zhi-Yong
中科院分区：
文献类型：
--
作者：
Gong, Chang-Yang;Shi, Shuai;Qian, Zhi-Yong
在这方面的贡献，生物降解和注射温敏聚（乙二醇）-聚（ε-己内酯）-聚（乙二醇）（PEG-PCL-PEG，PECE）水凝胶系统成功地制备了碱性成纤维细胞生长因子（bFGF）的抗原输送。bFGF包封的PECE水凝胶系统（bFGF-水凝胶）在环境温度下是可注射的自由流动的溶胶，并且在生理温度下形成非流动的凝胶，充当抗原储库。细胞毒性实验结果表明，该水凝胶可作为一种安全的载体，并能在体外较长时间内释放bFGF。另外，bFGF包封在水凝胶中后，其免疫原性明显提高。bFGF-水凝胶产生的强体液免疫维持超过14周。因此，制备的bFGF负载PECE水凝胶可能具有作为蛋白抗原的新型疫苗佐剂的巨大潜力。
In this contribution, a biodegradable and injectable thermosensitive poly(ethylene glycol)-poly(epsilon-caprolactone)-poly(ethylene glycol) (PEG-PCL-PEG, PECE) hydrogel system was successfully prepared for basic fibroblastic growth factor (bFGF) antigen delivery. bFGF encapsulated PECE hydrogel system (bFGF-hydrogel) is an injectable free-flowing sol at ambient temperature, and forms a non-flowing gel at physiological temperature acting as antigen depot. Furthermore, the cytotoxicity results showed that the PECE hydrogel could be regarded as a safe carrier, and bFGF could be released from the hydrogel system in an extended period in vitro. Otherwise, the immunogenicity of bFGF was improved significantly after encapsulated into the hydrogel. Strong humoral immunity created by bFGF-hydrogel was maintained for more than 14 weeks. Therefore, the prepared bFGF loaded PECE hydrogel might have great potential as a novel vaccine adjuvant for protein antigen.