Resting microglial motility is independent of synaptic plasticity in mammalian brain
Resting microglial motility is independent of synaptic plasticity in mammalian brain
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DOI：
10.1152/jn.01210.2007
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发表时间：
2008-04-01
影响因子：
2.5
通讯作者：
Zhuo, Min
中科院分区：
文献类型：
--
作者：
Wu, Long-Jun;Zhuo, Min
小胶质细胞因其在脑损伤和感染中的作用而闻名。然而，到目前为止，还没有关于静息小胶质细胞的功能属性。在这里，我们对急性海马切片中绿色荧光蛋白标记的小胶质细胞进行了同步电生理和延时共聚焦成像的结合。与CA1神经元相比，小胶质细胞没有自发或诱发的突触电流。谷氨酸和gaba诱导的小胶质细胞电流/趋化性均未检测到。强强电刺激诱发CA1长期增强的Schaffer-collateral通路不影响小胶质细胞的运动。我们的研究结果表明，小胶质细胞在大脑中具有高度的神经元保护功能，而不是突触可塑性。
Microglia are well known for their roles in brain injuries and infections. However, there is no function attributes to resting microglia thus far. Here we performed a combination of simultaneous electrophysiology and time-lapse confocal imaging in green fluorescent protein-labeled microglia in acute hippocampal slices. In contrast to CA1 neurons, microglia showed no spontaneous or evoked synaptic currents. Neither glutamate-nor GABA-induced current/chemotaxis of microglia was detected. Strong tetanic stimulation of Schaffer-collateral pathways that induce CA1 long-term potentiation did not affect microglial motilities. Our results suggest that microglia are highly reserved for neuronal protective function but not synaptic plasticity in the brain.