The intermediate-sized filaments in rat kangaroo PtK2 cells. I. Morphology in situ.
The intermediate-sized filaments in rat kangaroo PtK2 cells. I. Morphology in situ.
复制标题
大鼠袋鼠 PtK2 细胞中的中等大小的丝。
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
K. Weber
中科院分区：
文献类型：
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作者：
W. Franke;C. Grund;M. Osborn;K. Weber
通过电子显微镜研究了大鼠袋鼠 PtK2 细胞的中等大小丝 (IF) 系统，该系统可以使用某些兔自身抗体和针对牛蹄前角蛋白的豚鼠抗体通过免疫荧光显微镜具体证明。该系统的特征性装饰性弯曲阵列在薄片和全细胞制备物中原位固定后显示，代表延伸穿过整个细胞质的 6 至 11 nm 粗细丝束，尽管在某些细胞中它们似乎富集在核周区域。虽然许多单独的 IF 在细胞质中被识别，但此类细丝横向聚集成束的趋势是其显着特征之一。在这些束形成中，一种由紧密堆积的 IF 在致密嗜锇基质中粘合在一起组成的形式尤其引人注目。在用秋水仙和/或细胞松弛素 B 处理的细胞中，IF 的外观和排列模式没有显着改变。还描述了 IF 与含有微丝的电缆和微管以及与膜结构的空间关系。 IF 的宽度不均匀，显示出未染色的、明显中空的核心，表明管状组织。许多 IF 显示出小的、有时周期性排列的侧向投影，这似乎与 IF 交联有关。与多核糖体的关联很常见。还检查了有丝分裂期间 IF 系统的变化。 IF 的结构细节以及它们作为参与细胞形状和细胞质结构控制的细胞骨架元件的可能作用，将结合来自其他细胞类型的各种中等大小的丝的数据进行讨论。强调了 PtK2 细胞的 IF 与前角蛋白丝聚集体的密切相似性。这表明PtK2细胞代表了在平衡的半角化状态下生长的上皮细胞系。
The system of the intermediate-sized filaments (IF) of rat kangaroo PtK2 cells which can be specifically demonstrated by immunofluorescence microscopy using certain rabbit autoantibodies and guinea pig antibodies against bovine hoof prekeratin has been studied by electron microscopy. The characteristic ornamental, curved arrays of this system are shown after fixation in situ in both thin sections and whole-cell-preparations to represent bundles of 6 to 11 nm thick filaments extending through the whole cytoplasm, although in some cells they appear to be enriched in the perinuclear region. While many individual IF are recognized in the cytoplasm the tendency of such filaments to aggregate laterally into bundles is one of their prominent features. Among such bundle formations one form that consists of tightly packed IF cemented together in a dense osmiophilic matrix is especially conspicious. The appearance and mode of arrangement of the IF is not significantly altered in cells treated with colcemid and/or cytochalasin B. Spatial relationships of IF with microfilament-containing cables and microtubules as well as with membranous structures are also described. IF are heterogeneous in width and reveal an unstained, apparently hollow core, indicative of a tubular organization. Many IF show small, sometimes periodically arranged lateral projections which seem to be involved in IF cross-linking. Associations with polyribosomes are common. The changes in the IF system during mitosis have also been examined. The structural details of the IF as well as their possible role as cytoskeletal elements involved in the control of cell shape and cytoplasmic architecture are discussed in relation to data on various intermediate-sized filaments from other cell types. The close similarity of the IF of PtK2 cells to aggregates of prekeratin filaments is emphasized. It is suggested that PtK2 cells represent an epithelial cell line growing in a state of balanced semi-keratinization.