Molecular typing of enterohemorrhagic Escherichia coli O157:H7 isolates in Japan by using pulsed-field gel electrophoresis
Molecular typing of enterohemorrhagic Escherichia coli O157:H7 isolates in Japan by using pulsed-field gel electrophoresis
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DOI：
10.1128/jcm.35.7.1675-1680.1997
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发表时间：
1997-07-01
影响因子：
9.4
通讯作者：
Watanabe, H
中科院分区：
文献类型：
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作者：
Izumiya, H;Terajima, J;Watanabe, H
采用脉冲场凝胶电泳（PFGE）对825株肠出血性大肠杆菌（EHEC） O157:H7分离株进行了分子分型，其中大部分分离株来自1996年5 ~ 8月在日本发生的19次暴发和608例散发病例，通过PFGE将EHEC O157:H7分离株分为6型（I ~ V型和ND型）和UT不可分型；5 ~ 6月7次暴发的50株分离株和散发感染患者的60株分离株显示出几乎相同的PFGE模式，22个DNA片段中只有1个不同，它们被归为I型；另外10次暴发的99株分离株和1996年初夏从Kinki地区散发感染患者中获得的156株分离株显示出相同的PFGE模式，表明它们来自一次大规模暴发。他们被归类为II型。仅携带stx2基因的IV型肠出血性大肠杆菌分离株于6月在一所小学引起了另一次暴发。另外两种类型（III型和V型）的肠出血性大肠杆菌分离株与此次暴发无关，但已在日本的几个地区分离到。ND型肠出血性大肠杆菌分离株包括多种类型，不能归类为上述任何一种类型，25株分离株由于其基因组dna降解而无法分析，并表示为UT。这些结果表明，具有多种PFGE类型的EHEC O157:H7菌株已在日本传播，并于1996年夏季在日本引起了多次暴发和散发感染。
Pulsed-field gel electrophoresis (PFGE) was applied for molecular typing of 825 enterohemorrhagic Escherichia coli (EHEC) O157:H7 isolates, most of which were from 19 outbreaks and 608 sporadic cases in Japan, mainly in May to August 1996, By PFGE, the EHEC O157:H7 isolates were classified into six types (type I to V and ND [nondescript]) and UT untypeable isolates, Fifty isolates from seven outbreaks in May to June and 60 isolates from patients with sporadic cases of infection showed almost identical PFGE patterns which differed in only 1 of 22 DNA fragments, They were classified into type I, Ninety-nine isolates from 10 other outbreaks and 156 isolates from patients in the Kinki area with sporadic cases of infection obtained in the early summer of 1996 showed identical PFGE patterns, suggesting that they were derived from one huge outbreak. They were classified into type II. Type IV EHEC isolates, which had only the stx2 gene, caused another outbreak in a primary school in June. EHEC isolates of two other types, types III and V, were not related to the outbreak but were isolated in several parts of Japan. ND EHEC isolates included a variety of patterns which could not be classified into either of the types mentioned above, Twenty-five isolates could not be analyzed due to degradation of their genomic DNAs and were represented as UT. These results indicate that EHEC O157:H7 strains with various PFGE types have already spread to Japan and caused the multiple outbreaks and sporadic infections in Japan in the summer of 1996.