Extract of Salvia przewalskii Repair Tissue Damage in Chronic Hypoxia Maybe through the RhoA-ROCK Signalling Pathway
Extract of Salvia przewalskii Repair Tissue Damage in Chronic Hypoxia Maybe through the RhoA-ROCK Signalling Pathway
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DOI：
10.1248/bpb.b19-00775
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发表时间：
2020-03-01
影响因子：
2
通讯作者：
Wu, Xinan
中科院分区：
文献类型：
--
作者：
Wang, Yafeng;Duo, Delong;Wu, Xinan
鼠尾草是一种传统的中草药，具有抗菌、抗病毒、抗氧化、抗血栓形成和抗抑郁等特性。然而，其主要活性成分及其抗缺氧作用尚不清楚。本研究探讨了普氏金丝桃的主要活性成分及其抗缺氧活性。采用高效液相色谱法对其主要有效成分进行了检测。在小鼠和大鼠缺氧预处理模型中检测了普氏弧菌的抗缺氧作用。结果表明，丹参素钠、迷迭香酸、石精酸、丹酚酸B、二氢丹参酮I、隐丹参酮、丹参酮I和丹参酮IIA 8种有效成分均表现出一定的抗缺氧作用。此外，普氏弧菌还能增强小鼠的抗缺氧能力，表现为延长小鼠急性缺氧预处理的生存时间，改善急性缺氧诱导的超氧化物歧化酶（SOD）和乳酸脱氢酶（LDH）活性的变化。此外，S. przewalskii还通过下调缺氧诱导因子-1 α (HIF-1 α)、增殖细胞核抗原（PCNA）、Bcl-2、CDK4、CyclinD1和P27Kip1以及抑制促炎细胞因子和rhoa - rho相关蛋白激酶（ROCK）信号通路来修复慢性缺氧时的组织损伤。我们的研究结果为przewalskii作为治疗高原肺动脉高压的一种有前景的药物的抗缺氧作用提供了新的见解。
Salvia przewalskii Maxim is a traditional Chinese herbal medicine and is known to have antibacterial, antiviral, anti-oxidant, anti-thrombotic and anti-depressant properties. However, the major active components of S. przewalskii and its anti-hypoxic effects are still unclear. This study probed the major active component and anti-hypoxic activity of S. przewalskii. The major active components of S. przewalskii were detected by HPLC. The anti-hypoxic effects of S. przewalskii were detected in mice and a rat model of hypoxic preconditioning. The results showed that there are eight active components, including sodium danshensu, rosmarinic acid, lithospermic acid, salvianolic acid B, dihydrotanshinone I, cryptotanshinone, tanshinone I and tanshinone IIA, and each component showed a certain anti-hypoxic effect. Moreover, S. przewalskii enhanced anti-hypoxia in mice, which was manifested as prolonged survival time in acute hypoxic preconditioning and the amelioration of acute hypoxia-induced changes in the activity of superoxide dismutase (SOD) and lactate dehydrogenase (LDH). In addition, S. przewalskii also repaired tissue damage in chronic hypoxia by downregulating hypoxia inducible factor-1 alpha (HIF-1 alpha), proliferating cell nuclear antigen (PCNA), Bcl-2, CDK4, CyclinD1 and P27Kip1 and inhibiting pro-inflammatory cytokines and the RhoA-Rho-associated protein kinase (ROCK) signalling pathway. Our findings provide new insight into the anti-hypoxic effect of S. przewalskii as a promising agent for high-altitude pulmonary hypertension treatment.