Mucocyte distribution and relationship to particle transport on the pseudolamellibranch gill of Crassostrea virginica (Bivalvia: Ostreidae)
Mucocyte distribution and relationship to particle transport on the pseudolamellibranch gill of Crassostrea virginica (Bivalvia: Ostreidae)
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DOI：
10.3354/meps137133
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发表时间：
1996-06-01
影响因子：
2.5
通讯作者：
Dufour, SC
中科院分区：
文献类型：
--
作者：
Beninger, PG;Dufour, SC
为了研究颗粒在双壳类假鳃分支鳃上的转运机制，我们测定了东方牡蛎（Crassostrea virginica）这个器官的黏液细胞分泌类型和分布，并与之前的内镜数据相关。1994年7月，从Shediac Bay （New Brunswick, Canada）收集了3只成年牡蛎，立即固定，然后解剖处理，使用一种修改的周期性酸-希夫-阿利新蓝方法进行组织学和整个黏液细胞定位。一种类型的粘细胞含有酸分泌粘多糖（AMPS），而另一种类型含有中性粘多糖（NMPS）。黏液细胞密度从政治波峰到波谷有明显的梯度；除了最前面的15个皱襞外，所有皱襞中每种黏液细胞类型的比例保持不变；15个最前面的皱襞AMPS比例增加。这些比例在纤毛鳃表面混合后会产生相对粘稠的酸为主的粘液。主要的丝槽含有相对较少的黏液细胞，排列在正中脊上。这种排列可以解释在这些细丝中观察到的粒子速度范围。本研究的结果符合不同类型双壳类鳃上粘液类型和功能的专门化模式，这取决于颗粒轨迹、输送表面结构和主导水流。
To investigate particle transport mechanisms on the bivalve pseudolamellibranch gill, the mucocyte secretion types and distribution were determined for this organ in the eastern oyster Crassostrea virginica, and related to previous endoscopic data. Three adult oysters were collected from Shediac Bay (New Brunswick, Canada) in July 1994, immediately fixed, then dissected and processed for histology and whole mount mucocyte mapping using a modification of the periodic acid-Schiff-alcian blue protocol. One type of mucocyte contained acid-secretion mucopolysaccharides (AMPS), while the other type contained neutral mucopolysaccharides (NMPS). A clear gradient in mucocyte density was observed from the plical crest to the trough; for all but the anteriormost 15 plicae the proportions of each mucocyte type remained constant; the 15 anteriormost plicae presented an increased proportion of AMPS. These proportions would produce a relatively viscous acid-dominant mucus after mixing on the ciliated gill surface. The principal filament troughs contained relatively few mucocytes, aligned on the median ridge. This arrangement could account for the observed range oi particle velocities in these filaments. The results of the present study conform to a pattern of specialization of mucus types and functions on the diverse types of bivalve gill, depending on particle trajectory, transporting surface architecture, and dominant current flow.