Experimental Characterisation of Fluid Mechanics in a Spinner Flask Bioreactor
Experimental Characterisation of Fluid Mechanics in a Spinner Flask Bioreactor
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DOI：
10.3390/pr2040753
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发表时间：
2014-12-01
期刊：
影响因子：
3.5
通讯作者：
Fouras, Andreas
中科院分区：
文献类型：
--
作者：
Ismadi, Mohd-Zulhilmi;Hourigan, Kerry;Fouras, Andreas
与传统的培养方法相比，旋转瓶生物反应器具有提供均匀培养环境的优势，已广泛应用于体外细胞培养过程中。然而，人们对这些生物反应器中的流场了解有限，并且尚未确定最佳培养条件。本文介绍了不同转速（10转/分至80转/分）和叶轮位置下旋转烧瓶内流场的实验特性。采用光学、非侵入性测量技术粒子图像测速（PIV）来描述流体流动，并计算与烧瓶内流动相关的应力和涡量。在叶轮最高位置的子午面处观察到最大的再循环结构，而在旋转烧瓶底部观察到最大的剪应力区域。本研究概述了旋转烧瓶在子午面和方位面上的流体结构。此外，本研究的结果给出了给定叶轮转速下应力范围的精确量化。这些结果提供了在许多已发表的细胞研究中使用的旋转烧瓶类型的生物力学特性的估计。
The spinner flask bioreactor has been widely used in in vitro cell culturing processes due to its superiority in providing a homogeneous culture environment compared to traditional culturing methods. However, there is limited understanding of the flow fields in these bioreactors, and optimum culture conditions are yet to be determined. This article presents the experimental characterization of the flow field within a spinner flask at varying speeds (10 RPM to 80 RPM) and impeller positions. An optical, non-invasive measurement technique, Particle Image Velocimetry (PIV), was employed to illustrate the fluid flow and calculate the stresses and vorticity associated with the flow within the flask. The largest recirculation structure was observed in the meridional plane at the highest impeller position while the highest shear stress region was observed at the base of the spinner flask. The study provides an overview of the fluid structure within the spinner flask in the meridional and azimuthal planes. Furthermore, the results presented in this study give an accurate quantification of the range of stresses for the given impeller speeds. These results provide estimates of the biomechanical properties within the type of spinner flask used in many published cell studies.