A Sensitive Biosensor for Bisphenol A Based on a Graphene–Poly–L–Lysine/Tyrosinase Biocomposite Film Electrode
A Sensitive Biosensor for Bisphenol A Based on a Graphene–Poly–L–Lysine/Tyrosinase Biocomposite Film Electrode
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DOI：
10.1166/nnl.2014.1767
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发表时间：
2014-04
影响因子：
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通讯作者：
Y. Qu;Nina Liao;Jianpeng Chen;Guishen Liu;Chunya Li
中科院分区：
文献类型：
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作者：
Y. Qu;Nina Liao;Jianpeng Chen;Guishen Liu;Chunya Li
合成了石墨烯-聚-L-赖氨酸纳米片（Gr-PLL）并对其进行了表征。将酪氨酸酶固定在聚羟基喹啉（Gr-PLL）纳米片修饰的玻碳电极上，制备了双酚A电流型生物传感器。用扫描电镜和电化学阻抗谱对所制备的生物传感器进行了表征。考察了影响该传感器传感性能的实验条件。该传感器对双酚A的线性响应范围为0.02 μM ~ 8.0 μM，检出限为0.01 μM（S/N = 3）。该传感器已成功地应用于塑料饮料瓶中痕量双酚A的测定。
Graphene–poly–L–lysine nanosheets (Gr–PLL) were synthesized and fully characterized. Tyrosinase was immobilized on the Gr–PLL nanosheets modified glassy carbon electrode which was employed as an amperometric biosensor for bisphenol A. The obtained biosensor was characterized with scanning electron microscopy and electrochemical impedance spectroscopy. Experimental conditions on the sensing performance of the as-prepared biosensor were investigated. The biosensor displayed a linear response to bisphenol A over a concentration range which spanned from 0.02 μM to 8.0 μM with a detection limit of 0.01 μM (S/N = 3). The biosensor was successfully applied to determine trace amounts of bisphenol A leaching from plastic drinking bottles.