Effects of Vitamin B2 Supplementation in Broilers Microbiota and Metabolome
Effects of Vitamin B2 Supplementation in Broilers Microbiota and Metabolome
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DOI：
10.3390/microorganisms8081134
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发表时间：
2020-08-01
期刊：
影响因子：
4.5
通讯作者：
De Cesare, Alessandra
中科院分区：
文献类型：
--
作者：
Biagi, Elena;Mengucci, Carlo;De Cesare, Alessandra
对肉鸡微生物群的研究为制定健康维护和提高生产性能的策略提供了广阔的前景。旨在调节微生物区系-宿主关系的营养策略可以改善鸡的免疫状态和代谢适应性。在这里，我们介绍了一项试验性试验的结果，旨在分析补充维生素B2的营养策略对罗斯308肉鸡的回肠、盲肠和窝产微生物区系的影响，以及对盲肠内容物代谢的影响。三组鸡分别饲喂对照日粮和添加两种不同剂量维生素B2的日粮。在生产周期的三个时间点，从不同的禽类群中采集回肠、盲肠和窝样。16S rRNA V3-V4区序列测定和核磁共振代谢组学用于研究微生物区系组成和包括短链脂肪酸在内的感兴趣代谢物浓度。补充维生素B2显著调节盲肠微生物区系，最高剂量更有效地增加促进健康的细菌群，包括双歧杆菌，从而提高盲肠环境中丁酸盐的产量，丁酸盐是一种众所周知的促进健康的代谢物。
The study of the microbiome in broiler chickens holds great promise for the development of strategies for health maintenance and performance improvement. Nutritional strategies aimed at modulating the microbiota-host relationship can improve chickens' immunological status and metabolic fitness. Here, we present the results of a pilot trial aimed at analyzing the effects of a nutritional strategy involving vitamin B2 supplementation on the ileum, caeca and litter microbiota of Ross 308 broilers, as well as on the metabolic profile of the caecal content. Three groups of chickens were administered control diets and diets supplemented with two different dosages of vitamin B2. Ileum, caeca, and litter samples were obtained from subgroups of birds at three time points along the productive cycle. Sequencing of the 16S rRNA V3-V4 region and NMR metabolomics were used to explore microbiota composition and the concentration of metabolites of interest, including short-chain fatty acids. Vitamin B2 supplementation significantly modulated caeca microbiota, with the highest dosage being more effective in increasing the abundance of health-promoting bacterial groups, includingBifidobacterium, resulting in boosted production of butyrate, a well-known health-promoting metabolite, in the caeca environment.