Immunoglobulins of camel (Camelus dromedarius) colostrum
Immunoglobulins of camel (Camelus dromedarius) colostrum
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DOI：
10.1016/s0021-9975(96)80049-1
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发表时间：
1996-04-01
影响因子：
0.8
通讯作者：
Woldehiwet, Z
中科院分区：
文献类型：
--
作者：
Azwai, SM;Carter, SD;Woldehiwet, Z
采用酸沉淀法、凝胶过滤法和快速蛋白液相色谱法（离子交换法）对单峰骆驼初乳中的免疫球蛋白G、M和A进行了鉴定。重链和轻链通过聚丙烯酰胺凝胶电泳和Western印迹鉴定。通过DEAE离子交换层析分离IgG亚类（IgG（1）、IgG（2）和IgG（3）），并且显示具有不同的电泳迁移率。用酶联免疫吸附试验（ELISA）测定骆驼伊加与其它种属伊加的交叉反应性。大部分的免疫球蛋白含量是IgG，但可识别为伊加的分子被检测和纯化。看来骆驼和牛一样，IgG是初乳的主要分泌免疫球蛋白。(C)1996年W.B.桑德斯有限公司
Immunoglobulins G, M and A were identified in dromedary camel colostra by acid precipitation, gel filtration and fast-protein liquid chromatography (ion exchange). Heavy and light chains were identified by polyacrylamide gel electrophoresis and Western blotting. IgG subclasses (IgG(1), IgG(2) and IgG(3)) were isolated by DEAE ion-exchange chromatography and shown to have different electrophoretic mobilities. Cross-reactivity of camel IgA with IgA of other species was determined by enzyme-linked immunosorbent assay (ELISA). Most of the immunoglobulin content was IgG, but a molecule identifiable as IgA was detected and purified. It would appear that in the camel, as in cattle, IgG is the major secretory immunoglobulin of colostrum. (C) 1996 W.B. Saunders Company Limited