KIDNEY BEAN LECTIN-INDUCED ESCHERICHIA-COLI OVERGROWTH IN THE SMALL-INTESTINE IS BLOCKED BY GNA, A MANNOSE-SPECIFIC LECTIN
KIDNEY BEAN LECTIN-INDUCED ESCHERICHIA-COLI OVERGROWTH IN THE SMALL-INTESTINE IS BLOCKED BY GNA, A MANNOSE-SPECIFIC LECTIN
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DOI：
10.1111/j.1365-2672.1993.tb02788.x
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发表时间：
1993-10-01
期刊：
影响因子：
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通讯作者：
BARDOCZ, S
中科院分区：
文献类型：
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作者：
PUSZTAI, A;GRANT, G;BARDOCZ, S
可逆的和剂量依赖性的增生性生长的小肠和加速上皮细胞的营业额所造成的饲料中含有菜豆凝集素（PHA）喂养大鼠的绒毛膜和/或细胞质中主要含有简单的，多聚甘露糖基化聚糖的比例增加的未成熟细胞。这些新的α-连接的甘露糖末端，特别是受损的上皮，促进了甘露糖敏感的1型菌毛和其他大肠菌群的机会性大肠杆菌优先粘附到糖萼上。因此，肠道的生长伴随着可逆的和PHA剂量依赖性的过度生长与大肠杆菌。正如从它们共同的碳水化合物特异性所预期的，在饮食中包含来自雪花莲（Galanthus nivalis）鳞茎的甘露糖特异性凝集素（GNA）显著降低了大肠杆菌过度生长的程度，但既不消除生长也不消除PHA对小肠造成的损伤。因此，GNA和可能的其他甘露糖特异性凝集素，特别是当用于预防模式时，可用于特异性阻断小肠中1型大肠杆菌的增殖。
The reversible and dose-dependent hyperplastic growth of the small intestine and accelerated epithelial cell turnover caused by feeding rats with diets containing kidney bean lectin (PHA) increased the proportion of immature cells on the villi whose membrane and/or cytoplasm contained mainly simple, polymannosylated glycans. These new alpha-linked mannosyl terminals, particularly of the damaged epithelium, facilitated the preferential adherence of opportunistic Escherichia coli with mannose-sensitive Type 1 fimbriae, and other coliforms, to the glycocalyx. Accordingly, the growth of the gut was accompanied by a reversible and PHA dose-dependent overgrowth with E.coli. As expected from their common carbohydrate specificity, the inclusion in the diet of the mannose-specific agglutinin from snowdrop (Galanthus nivalis) bulbs (GNA) significantly reduced the extent of E.coli overgrowth, but abolished neither the growth nor the damage caused by PHA to the small intestine. Thus, GNA and perhaps other mannose-specific lectins, especially when used in a preventive mode, can be used to specifically block the proliferation of Type 1 E.coli in the small intestine.