Oral health status of children in relation to the dental anxiety of their parents.
Oral health status of children in relation to the dental anxiety of their parents.
复制标题
儿童口腔健康状况与父母牙科焦虑的关系。
DOI：
10.1016/j.jcis.2005.12.043
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
S. Lahti
中科院分区：
文献类型：
--
作者：
H. Tuutti;S. Lahti
为了研究脂质体与蛋白质之间的相互作用，使用石英晶体微天平（QCM）通过化学结合和/或生物亲和性将完整的脂质体固定在金属平面载体上。经羧基硫醇自组装单层（SAM）修饰的QCM加入脂质体溶液后，石英晶体的共振频率明显降低。根据制备的脂质体的大小以及引入氨基偶联时脂质体的激活时间来判断完整脂质体在SAM上的吸附质量是否稳定，在最佳条件下脂质体的电极覆盖率为69±8%。当在氨基偶联脂质体层上使用亲和素-生物素结合时，脂质体的固定最终达到了电极表面168%的覆盖率。根据脂质体固定的QCM频率的变化，也成功地检测到变性蛋白。从牛身上吸附的变性碳酸酐酶在固定脂质体上的吸附质量在盐酸胍浓度下显示出一个特征峰，该峰对应于蛋白质的熔融球状状态，尽管在失活的SAM上吸附的质量单调增加。
To study the interaction between liposomes and proteins, intact liposomes were immobilized on a metal planar support by chemical binding and/or bioaffinity using a quartz crystal microbalance (QCM). A large decrease in the resonance frequency of quartz crystal was observed when the QCM, modified by a self-assembled monolayer (SAM) of carboxythiol, was added to liposome solutions. The stable chemical immobilization of intact liposomes onto SAM was judged according to the degree with which adsorbed mass depended on the prepared size of liposomes, as well as on the activation time of SAMs when amino-coupling was introduced, where the liposome coverage of electrodes was 69±8% in optimal conditions. When avidin–biotin binding was used on amino-coupling liposome layers, liposome immobilization finally reached 168% coverage of the electrode surface. Denatured protein was also successfully detected according to the change in the frequency of the liposome-immobilized QCM. The adsorbed mass of denatured carbonic anhydrase from bovine onto immobilized liposomes showed a characteristic peak at a concentration of guanidine hydrochloride that corresponded to a molten globule-like state of the protein, although the mass adsorbed onto deactivated SAM increased monotonously.