INTERFERENCE BY LIPIDS IN THE DETERMINATION OF PROTEIN USING BICINCHONINIC ACID
INTERFERENCE BY LIPIDS IN THE DETERMINATION OF PROTEIN USING BICINCHONINIC ACID
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DOI：
10.1016/0003-2697(86)90318-0
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发表时间：
1986-11-15
影响因子：
2.9
通讯作者：
FANESTIL, DD
中科院分区：
文献类型：
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作者：
KESSLER, RJ;FANESTIL, DD
联苯胆酸是测定蛋白质的分析方法的基础。该试剂在碱性环境中与Cu2+形成紫色络合物，是监测蛋白质与碱性Cu2+反应生成Cu2+的基础。据报道，与Lowry技术相比，这种蛋白质测定方法对许多常见的干扰化合物具有更大的耐受性。然而，我们发现，当常见的膜磷脂被包括在检测中时，二辛酸技术会错误地产生高值的蛋白质。磷脂在比辛酸存在下产生与蛋白质产生的吸收峰相似的吸收峰。吸光度与浓度呈线性关系，但不同磷脂的斜率不同。磷脂和蛋白质的联合吸收不是严格相加的。结果表明，样品中有相当数量的脂类存在会导致用比胆碱酸法分析蛋白质时产生明显的误差。
Bicinchoninic acid forms the basis of an analytical method for the determination of protein. The reagent produces a purple complex with cuprous ion (Cu+) in an alkaline environment and is the basis for the monitoring of cuprous ions produced in the reactions of proteins with alkaline is the basis for the monitoring of cuprous ions produced in the reactions of proteins with alkaline Cu2+. This method of protein determination was reported to have greater tolerance to many commonly encountered interfering compounds, when compared to the Lowry technique. However, we have found the bicinchoninic acid technique to produce erroneously high values for protein when common membrane phospholipids were included in the assay. Phospholipids in the presence of bicinchoninic acid produced an absorbance peak similar to that produced by protein. This absorbance was linear with concentration, however, the slope varied for individual phospholipids. The combined absorption of phospholipid and protein was not strictly additive. The results indicate that the presence of appreciable quantities of lipid in samples can cause significant error in the analysis of protein by the bicinchoninic acid procedure.