L-arginine stimulates insulin secretion from the pancreas of normal and diabetic rats
L-arginine stimulates insulin secretion from the pancreas of normal and diabetic rats
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DOI：
10.1007/s007260170028
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发表时间：
2001-01-01
期刊：
影响因子：
3.5
通讯作者：
Parvez, H
中科院分区：
文献类型：
--
作者：
Adeghate, E;Ponery, AS;Parvez, H
已有报道表明，一氧化氮(NO)可刺激正常大鼠胰腺葡萄糖诱导的胰岛素分泌，但NO供体L精氨酸对糖尿病大鼠胰腺胰岛素分泌的影响尚不清楚。取正常大鼠和糖尿病大鼠的胰腺组织，在含100mM L-精氨酸的Krebs液中孵育45min。随后用放射免疫分析技术分析上清液中的胰岛素含量。L-精氨酸可使糖尿病大鼠胰腺胰岛素分泌显著增加。糖尿病大鼠胰腺分泌的胰岛素在数量上低于正常大鼠，但不显著低于正常大鼠。综上所述，一氧化氮供体L精氨酸可促进糖尿病大鼠胰腺胰岛素的分泌。
Several reports have shown that nitric oxide (NO) stimulates glucose-induced insulin secretion in the pancreas of normal rat but the effect of L-arginine (a NO donor) on insulin secretion from the pancreas of diabetic pancreas is unknown. Fragments of pancreatic tissue from normal and diabetic rats were incubated for 45 min in Krebs solution containing 100 mM L-arginine. The supernatant was subsequently analyzed for the insulin content using radioimmunoassay technique. L-arginine evoked large increases in insulin secretion from the pancreas of diabetic rat. The insulin secreted from the pancreas of diabetic rat was numerically but not significantly lower compared to that of normal rat pancreas. In conclusion, L-arginine, a nitric oxide donor stimulates insulin secretion from the pancreas of diabetic rats.