A newly developed age estimation method based on CpG methylation of teeth-derived DNA using real-time methylation-specific PCR
A newly developed age estimation method based on CpG methylation of teeth-derived DNA using real-time methylation-specific PCR
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DOI：
10.2334/josnusd.20-0138
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发表时间：
2021-01-01
影响因子：
1.9
通讯作者：
Aizawa, Shin
中科院分区：
文献类型：
--
作者：
Kondo, Masahiro;Aboshi, Hirofumi;Aizawa, Shin
不明身份身体的年龄估计在法医和犯罪现场很重要。越来越多的证据表明，从体液中分离的人类基因组中的DNA甲基化随着年龄的变化而变化。大部分数据是通过焦磷酸测序获得的。在法医领域，一种简单、快速的。并且需要经济的方法来评估各种类型的样品的年龄。在这项研究中，开发了一种基于实时甲基化特异性聚合酶链式反应(MSP)提取的牙齿DNA甲基化水平来估计年龄的方法。ELOVL2上游区域的CpG岛被选为靶点，该岛在血液样本中被认为是一个经过验证的生物标志物。CpG甲基化水平与年龄高度相关(r=0.843，n=29)。年龄相关的DNA甲基化水平的增加不受性别差异的影响。此外，基于CpG岛甲基化状态的简单回归模型具有中等精度，实际年龄与预测年龄之间的平均绝对偏差为8.94岁。这些结果表明，实时MSP可以作为一种新的工具来进行法医场景中身份不明身体的年龄预测。
Age estimation of unidentified bodies is important in forensic medicine and crime scenes. There is accumulating evidence that DNA methylation in the human genome isolated from body fluids changes with age. Most of the data have been obtained by pyrosequencing. In the forensic field, a simple, quick. and economical method is required to evaluate the age of various types of samples. In this study, an age estimation method based on methylation levels of DNA extracted from teeth using real-time methylation-specific PCR (MSP) was developed. The CpG island in the upstream region of ELOVL2, which is known as a validated biomarker in blood samples, was selected as a target site. The CpG methylation levels highly correlated with age (r=0.843, n = 29). Age-related increase in DNA methylation levels was not affected by sex differences. In addition, the simple regression model based on methylation status of the CpG island exhibited moderate accuracy with a mean absolute deviation between chronological age and predicted age of 8.94 years. The results imply that real-time MSP can be a new tool to perform age prediction of unidentified bodies in forensic scenes.