Light-powered Escherichia coli cell division for chemical production
Light-powered Escherichia coli cell division for chemical production
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DOI：
10.1038/s41467-020-16154-3
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发表时间：
2020-05-08
影响因子：
16.6
通讯作者：
Chen, Xiulai
中科院分区：
文献类型：
--
作者：
Ding, Qiang;Ma, Danlei;Chen, Xiulai
细胞分裂可以扰乱工业微生物的代谢性能。细胞分裂的C期从DNA复制的开始到终止，而D期是细菌的分裂过程。在这里，我们首先缩短E的C和D周期。分别通过蓝光和近红外光激活来控制核糖核苷酸还原酶NrdAB和分裂蛋白FtsZA的表达。比表面积增加到3.7 μ m ~（-1），乙偶姻效价增加到67.2g·L ~（-1）。其次，我们延长了E的C和D周期。分别通过蓝光激活-抑制和近红外（NIR）光激活调节核糖核苷酸还原酶NrdA和分裂蛋白抑制剂苏拉的表达，构建了表达量为10000 μ g/L的重组质粒。它将细胞体积提高到52.6 μ m（3），聚（乳酸-co-3-羟基丁酸酯）滴度提高到14.31g·L-1。因此，基于光遗传学的细胞分裂调控策略可以提高微生物细胞工厂的效率。操纵控制微生物形状的基因可以影响生物生产。本文采用光遗传学方法实现了大肠杆菌的动态形态工程。大肠杆菌复制和分裂，并显示乙偶姻和聚（乳酸-co-3-羟基丁酸酯）的产量增加。
Cell division can perturb the metabolic performance of industrial microbes. The C period of cell division starts from the initiation to the termination of DNA replication, whereas the D period is the bacterial division process. Here, we first shorten the C and D periods of E. coli by controlling the expression of the ribonucleotide reductase NrdAB and division proteins FtsZA through blue light and near-infrared light activation, respectively. It increases the specific surface area to 3.7 mu m(-1) and acetoin titer to 67.2g.L-1. Next, we prolong the C and D periods of E. coli by regulating the expression of the ribonucleotide reductase NrdA and division protein inhibitor SulA through blue light activation-repression and near-infrared (NIR) light activation, respectively. It improves the cell volume to 52.6 mu m(3) and poly(lactate-co-3-hydroxybutyrate) titer to 14.31g.L-1. Thus, the optogenetic-based cell division regulation strategy can improve the efficiency of microbial cell factories. Manipulation of genes controlling microbial shapes can affect bio-production. Here, the authors employ an optogenetic method to realize dynamic morphological engineering of E. coli replication and division and show the increased production of acetoin and poly(lactate-co-3-hydroxybutyrate).