Production of colony-stimulating factor in mitogen-stimulated lymphocyte cultures.
Production of colony-stimulating factor in mitogen-stimulated lymphocyte cultures.
复制标题
在有丝分裂原刺激的淋巴细胞培养物中产生集落刺激因子。
作者：
John W. Parker;Donald Metcalf
此外，植物血凝素，美洲商陆有丝分裂原，刀豆球蛋白A或细菌脂多糖的小鼠脾培养物强烈刺激集落刺激因子（CSF），体液调节粒细胞生成和巨噬细胞形成的生产。去除巨噬细胞的柱过滤脾细胞表现出相似的反应。培养的巨噬细胞单层未能产生升高的CSF水平时，刺激PHA或PWM。结果表明，活化的T-和B-淋巴细胞都可以产生大量的CSF，提供了一种可能的机制，刺激巨噬细胞形成的慢性免疫或炎症反应。
The addition of phytohemagglutinin, pokeweed mitogen, concanavalin A or bacterial lipopolysaccharide to mouse spleen cultures strongly stimulated production of colony-stimulating factor (CSF), the humoral regulator of granulopoiesis and macrophage formation. Column-filtered spleen cells depleted of macrophages exhibited similar responses. Cultures of macrophage monolayers failed to produce elevated levels of CSF when stimulated by PHA or PWM. The results suggest that activated T- and B-lymphocytes can both produce significant amounts of CSF, providing a possible mechanism for stimulating macrophage formation in chronic immunologic or inflammatory responses.