T cells of different developmental stages differ in sensitivity to apoptosis induced by extracellular NAD.
T cells of different developmental stages differ in sensitivity to apoptosis induced by extracellular NAD.
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DOI：
10.1080/10446670310001593514
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发表时间：
2002-12-01
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影响因子：
--
通讯作者：
Koch-Nolte, Friedrich
中科院分区：
文献类型：
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作者：
Haag, Friedrich;Freese, Dunja;Koch-Nolte, Friedrich
细胞外核苷酸如ATP和NAD可以深刻影响淋巴细胞、巨噬细胞和其他细胞的功能。我们最近的研究表明，细胞外NAD通过一种涉及细胞表面分子adp核糖基化的机制诱导幼稚T细胞的快速凋亡。在本文中，我们描述了不同发育阶段的T细胞对nadd诱导的凋亡的敏感性不同。胸腺细胞比周围淋巴结T细胞更不敏感，新激活的细胞比静止的细胞更耐药。对nadd诱导的凋亡的敏感性通常与adp -核糖基转移酶ART2.2的表达相关，该酶在胸腺细胞上不表达，激活后会从外周T细胞脱落。我们的研究结果表明，nad诱导的细胞凋亡并不在T细胞的胸腺选择中起作用，而是在免疫反应中通过阻止不需要的旁观者T细胞的激活发挥作用，从而可能参与自身免疫的控制。
Extracellular nucleotides such as ATP and NAD can profoundly affect the functions of lymphocytes, macrophages, and other cells. We have recently shown that extracellular NAD induces rapid apoptosis in naive T cells by a mechanism involving the ADP-ribosylation of cell surface molecules. In the present paper, we describe that T cells of different developmental stages differ in their sensitivity to NAD-induced apoptosis. Thymocytes were less susceptible than peripheral lymph node T cells, and freshly activated cells were more resistant than resting cells. Sensitivity to NAD-induced apoptosis generally correlated with expression of the ADP-ribosyltransferase ART2.2, which is not expressed on thymocytes and shed from peripheral T cells upon activation. Our findings suggest that NAD-induced apoptosis does not play a role during thymic selection of T cells, but rather may play a role by preventing the activation of unwanted bystander T cells during an immune response, and thus may participate in the control of autoimmunity.