SEQUENCE-SPECIFIC DNA-BINDING BY THE C-MYC PROTEIN
SEQUENCE-SPECIFIC DNA-BINDING BY THE C-MYC PROTEIN
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DOI：
10.1126/science.2251503
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发表时间：
1990-11-23
期刊：
影响因子：
56.9
通讯作者：
WEINTRAUB, H
中科院分区：
文献类型：
--
作者：
BLACKWELL, TK;KRETZNER, L;WEINTRAUB, H
虽然已经知道c-Myc蛋白与随机DNA序列结合已有一段时间，但尚未检测到序列特异性结合活性。在其羧基末端，c-Myc含有碱性-螺旋-环-螺旋（bHLH）基序，其对于二聚化和特异性DNA结合是重要的，如对于其他bHLH蛋白家族成员所证明的。在这些研究中，大多数bHLH蛋白质结合到含有CA-TG共识的位点。本研究采用选择和扩增结合序列（SAAB）印记技术来鉴定c-Myc识别的DNA序列。纯化的人c-Myc的羧基末端片段含有bHLH结构域，在体外以序列特异性方式与序列CACGTG结合。这些结果表明，Myc家族蛋白的一些生物学功能是通过序列特异性DNA结合来实现的，这种结合是由蛋白的羧基末端区域介导的。
While it has been known for some time that the c-Myc protein binds to random DNA sequences, no sequence-specific binding activity has been detected. At its carboxyl terminus, c-Myc contains a basic-helix-loop-helix (bHLH) motif, which is important for dimerization and specific DNA binding, as demonstrated for other bHLH protein family members. Of those studied, most bHLH proteins bind to sites that contain a CA--TG consensus. In this study, the technique of selected and amplified binding-sequence (SAAB) imprinting was used to identify a DNA sequence that was recognized by c-Myc. A purified carboxyl-terminal fragment of human c-Myc that contained the bHLH domain bound in vitro in a sequence-specific manner to the sequence, CACGTG. These results suggest that some of the biological functions of Myc family proteins are accomplished by sequence-specific DNA binding that is mediated by the carboxyl-terminal region of the protein.