HepatoProteomics: Applying proteomic technologies to the study of liver function and disease
HepatoProteomics: Applying proteomic technologies to the study of liver function and disease
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DOI：
10.1002/hep.21318
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发表时间：
2006-08-01
期刊：
影响因子：
13.5
通讯作者：
Katze, Michael G.
中科院分区：
文献类型：
--
作者：
Diamond, Deborah L.;Proll, Sean C.;Katze, Michael G.
人类基因组序列信息的丰富性，加上机器人、纳米技术、质谱和信息系统的技术进步，已经产生了一种被称为功能基因组学的科学研究方法。通过使用这些技术在全球范围内调查基因表达和蛋白质生产，功能基因组学的目标是将生物学功能分配给目前在生理学中具有未知作用的基因。这种方法在疾病研究中具有特别的吸引力，它可以揭示以前未知的基因和直接参与疾病进展的分子途径的功能。有了这些知识，可能会改进诊断技术，预后能力和新的治疗方法。在这方面，蛋白质组学技术的不断发展导致蛋白质组学研究在许多研究领域的影响越来越大，肝脏病学也不例外。我们的实验室在这一领域非常活跃，将基因组学和蛋白质组学技术应用于几个系统中病毒-宿主相互作用的分析，包括丙型肝炎病毒（HCV）感染和HCV相关肝病的研究。由于蛋白质组学技术对许多肝病学家（以及几乎所有其他人）来说都是陌生的，本文将概述蛋白质组学方法和技术，并描述它们如何用于研究肝功能和疾病。
The wealth of human genome sequence information now available, coupled with technological advances in robotics, nanotechnology, mass spectrometry, and information systems, has given rise to a method of scientific inquiry known as functional genomics. By using these technologies to survey gene expression and protein production on a near global scale, the goal of functional genomics is to assign biological function to genes with currently unknown roles in physiology. This approach carries particular appeal in disease research, where it can uncover the function of previously unknown genes and molecular pathways that are directly involved in disease progression. With this knowledge may come improved diagnostic techniques, prognostic capabilities, and novel therapeutic approaches. In this regard, the continuing evolution of proteomic technologies has resulted in an increasingly greater impact of proteome studies in many areas of research and hepatology is no exception. Our laboratory has been extremely active in this area, applying both genomic and proteomic technologies to the analysis of virus-host interactions in several systems, including the study of hepatitis C virus (HCV) infection and HCV-associated liver disease. Since proteomic technologies are foreign to many hepatologists (and to almost everyone else), this article will provide an overview of proteomic methods and technologies and describe how they are being used to study liver function and disease.