Diets deficient in indispensable amino acids rapidly decrease the concentration of the limiting amino acid in the anterior piriform cortex of rats
Diets deficient in indispensable amino acids rapidly decrease the concentration of the limiting amino acid in the anterior piriform cortex of rats
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DOI：
10.1093/jn/134.9.2365
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发表时间：
2004-09-01
影响因子：
4.2
通讯作者：
Gietzen, DW
中科院分区：
文献类型：
--
作者：
Koehnle, TJ;Russell, MC;Gietzen, DW
长期以来，人们都知道缺乏一种必需氨基酸的饮食会抑制老鼠的食物摄入。饮食缺乏的检测发生在前梨状皮质（APC）。最近的研究表明，对氨基酸缺乏的反应只需15分钟就能形成，但很少有数据表明APC中氨基酸浓度与这种快速反应之间存在关联。本研究的目的是在行为相关的时间框架内测量APC中氨基酸的浓度。先给大鼠喂基础饮食7-10 d，然后给它们饲喂氨基酸对照或缺乏氨基酸的试验饮食。缺乏苏氨酸和亮氨酸的饲粮分别在30分钟内消耗了APC中的苏氨酸和亮氨酸浓度。对照组饮食和缺乏必需氨基酸甘氨酸的饮食没有导致氨基酸消耗。结合前人的研究，本研究结果表明，APC中必需氨基酸浓度的下降可能是识别饲粮氨基酸缺乏的初始感官信号。
Diets deficient in an indispensable amino acid have long been known to suppress food intake in rats. Detection of dietary deficiency takes place in the anterior piriform cortex (APC). Recent studies showed that the response to amino acid deficiency takes as little as 15 min to develop, but few data exist to correlate the concentration of amino acids in the APC with this rapid response. The purpose of this study was to measure the concentration of amino acids in the APC in a behaviorally relevant time frame. Rats were preconditioned by consumption of a basal diet for 7-10 d, and then given a test diet with either a control or deficient amino acid profile. Both the threonine- and leucine-deficient diets reliably depleted threonine and leucine concentration in the APC within 30 min, respectively. The control diets and a diet lacking the dispensable amino acid glycine did not lead to amino acid depletion. In combination with previous studies, the present results show that the decrease in the concentration of indispensable amino acids in the APC may be the initial sensory signal for recognition of dietary amino acid deficiency.