Lipidomics of intact mitochondria by MALDI-TOF/MS
Lipidomics of intact mitochondria by MALDI-TOF/MS
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DOI：
10.1194/jlr.d026203
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发表时间：
2012-07-01
影响因子：
6.5
通讯作者：
Corcelli, Angela
中科院分区：
文献类型：
--
作者：
Angelini, Roberto;Vitale, Rita;Corcelli, Angela
本文介绍了一种用MALDI-TOF质谱法对分离的完整线粒体进行脂质分析的简单快速方法。从牛心脏和酵母中分离的线粒体建立并验证了新的脂质分析方法。将线粒体悬浮液直接涂在目标物上，干燥后，覆盖一层薄薄的9-氨基吖啶基质溶液。本程序获得的脂质谱包含以前通过TLC和/或质谱分析分离线粒体脂质提取物获得的所有峰。这种新方法可以快速、简单、精确和准确地分析膜脂，只需要少量的分离细胞器；它还用反硝化副球菌的完整膜进行了测试，以确定其与当今线粒体的进化联系。该方法对其他细胞组分和分离细胞器的脂质分析具有普遍的有效性。Angelini， R.， R. Vitale， V. A. Patil， T. Cocco， B. Ludwig， M. L. Greenberg和A. Corcelli。MALDI-TOF/MS对完整线粒体的脂质组学研究。[j] .油脂杂志。2012。53: 1417 - 1425。
A simple and fast method of lipid analysis of isolated intact mitochondria by means of MALDI-TOF mass spectrometry is described. Mitochondria isolated from bovine heart and yeast have been employed to set up and validate the new method of lipid analysis. The mitochondrial suspension is directly applied over the target and, after drying, covered by a thin layer of the 9-aminoacridine matrix solution. The lipid profiles acquired with this procedure contain all peaks previously obtained by analyzing the lipid extracts of isolated mitochondria by TLC and/or mass spectrometry. The novel procedure allows the quick, simple, precise, and accurate analysis of membrane lipids, utilizing only a tiny amount of isolated organelle; it has also been tested with intact membranes of the bacterium Paracoccus denitrificans for its evolutionary link to present-day mitochondria.(Jlr) The method is of general validity for the lipid analysis of other cell fractions and isolated organelles. Angelini, R., R. Vitale, V. A. Patil, T. Cocco, B. Ludwig, M. L. Greenberg, and A. Corcelli. Lipidomics of intact mitochondria by MALDI-TOF/MS. J. Lipid Res. 2012. 53: 1417-1425.