Intrathecal Administration of Nanoclusters for Protecting Neurons against Oxidative Stress in Cerebral Ischemia/Reperfusion Injury
Intrathecal Administration of Nanoclusters for Protecting Neurons against Oxidative Stress in Cerebral Ischemia/Reperfusion Injury
复制标题
鞘内注射纳米簇保护神经元免受脑缺血/再灌注损伤中的氧化应激
DOI：
10.1021/acsnano.9b06780
复制
发表时间：
2019-11-01
期刊：
影响因子：
17.1
通讯作者：
Cai, Weibo
中科院分区：
文献类型：
--
作者：
Li, Shiyong;Jian, Dawei;Cai, Weibo
氧化应激是脑缺血再灌注损伤的重要机制之一。高度期望具有高脑积累的抗氧化剂来帮助预防脑I/R损伤。在此，鞘内注射聚氧乙烯酸（POM）纳米簇作为纳米抗氧化剂，具有优先脑摄取，适用于脑I/R损伤的神经元保护。使用强大的正电子发射断层扫描成像技术，对大脑中纳米抗氧化剂的摄取进行了非侵入性和实时监测。我们的研究结果表明，POM纳米簇鞘内注射后迅速到达缺血半暗带，并有效地清除活性氧（ROS），抑制氧化应激。脑缺血再灌注模型大鼠脑梗死范围缩小，神经功能恢复。作为概念验证，鞘内注射纳米抗氧化剂是改善脑I/R损伤的极好治疗策略。
Oxidative stress is one of the important mechanisms in cerebral ischemia/reperfusion (I/R) injury. Antioxidants with high brain accumulation are highly desired to help prevent cerebral I/R injury. Herein, intrathecal injection of polyoxometalate (POM) nano clusters as nano-antioxidants with preferential brain uptake were applied for neuronal protection in cerebral I/R injury. Using powerful positron emission tomography imaging, the uptake of nano-antioxidants in the brain was non-invasively and real-timely monitored. Our results demonstrated that POM nanoclusters rapidly reached the ischemic penumbra after intrathecal injection and effectively scavenged reactive oxygen species (ROS) for inhibiting oxidative stress. The infarct size was reduced, and neurological function was restored in cerebral I/R injury rat models. As a proof-of-concept, the intrathecal injection of nano-antioxidants is an excellent therapeutic strategy to ameliorate cerebral I/R injury.