Molecular definition of high-resolution multicolor banding probes: First within the human DNA sequence anchored FISH banding probe set
Molecular definition of high-resolution multicolor banding probes: First within the human DNA sequence anchored FISH banding probe set
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DOI：
10.1369/jhc.2008.950550
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发表时间：
2008-05-01
影响因子：
3.2
通讯作者：
Kosyakova, Nadezda
中科院分区：
文献类型：
--
作者：
Weise, Anja;Mrasek, Kristin;Kosyakova, Nadezda
荧光原位杂交（FISH）显带方法是准确表征人类基因组中简单、复杂甚至隐性染色体畸变的标准方法。最常用的FISH显带技术是荧光显带法，也缩写为m带、MCB或其全基因组变体多MCB（mMCB）。MCB允许在GTG带和子带水平区分染色体区域特异性区域，并基于区域特异性显微切割文库，沿染色体产生变化的荧光强度比沿着。后者用于将不同的假彩色分配给特定的染色体区域。在这里，我们提出了第一个细菌人工染色体（BAC）阵列比较基因组杂交（aCGH）映射，全面，全基因组的人类MCB探针集。所有169个区域特异性显微切割文库的大小和重叠区域进行了详细的表征。在MCB技术表征染色体断裂总结的独特可能性中，一个FISH实验中的点现在通过在BAC水平锚定在人DNA序列内的特征来补充。
Fluorescence in situ hybridization (FISH) banding approaches are standard for the exact characterization of simple, complex, and even cryptic chromosomal aberrations within the human genome. The most frequently applied FISH banding technique is the multicolor banding approach, also abbreviated as m-band, MCB, or in its whole genomic variant multitude MCB (mMCB). MCB allows the differentiation of chromosome region-specific areas at the GTG band and sub-band level and is based on region-specific microdissection libraries, producing changing fluorescence intensity ratios along the chromosomes. The latter are used to assign different pseudocolors to specific chromosomal regions. Here we present the first bacterial artificial chromosome (BAC) array comparative genomic hybridization (aCGH) mapped, comprehensive, genome-wide human MCB probe set. All 169 region-specific microdissection libraries were characterized in detail for their size and the regions of overlap. In the unique possibilities of the MCB technique to characterize chromosomal break-summary, points in one FISH experiment are now complemented by the feature of being anchored within the human DNA sequence at the BAC level.