The identification of inducible cytoplasmic/nuclear carbohydrate-binding proteins urges to develop novel concepts about the role of plant lectins.
The identification of inducible cytoplasmic/nuclear carbohydrate-binding proteins urges to develop novel concepts about the role of plant lectins.
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DOI：
10.1023/b:glyc.0000038291.67527.a5
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发表时间：
2004-01-01
影响因子：
3
通讯作者：
Peumans, Willy J
中科院分区：
文献类型：
--
作者：
Van Damme, Els J M;Lannoo, Nausicaa;Peumans, Willy J
在过去的几年中，已经提出了令人信服的证据表明细胞质/核植物凝集素的存在，这些植物凝集素在正常植物中无法检测到，但只有在施加明确的胁迫条件时才会被诱导。由于表达调节和亚细胞定位都表明这些“非经典凝集素”是在植物细胞内作为特定蛋白质-碳水化合物相互作用的介质发挥重要生理作用的良好候选者，因此对这些发现对植物凝集素作用新概念发展的影响进行了关键评估。基于对来自烟叶的茉莉酸诱导的几丁质结合凝集素和来自水稻的盐/茉莉酸诱导的叶凝集素的生化、分子和进化数据的分析，得出的结论是，这些凝集素最有可能与位于植物细胞的细胞质/核区室内的内源聚糖相互作用。提出了几种工作机制来解释这些诱导型凝集素如何在应激细胞中发挥重要的调节或结构作用。此外，还解决了新发现的诱导型凝集素与其“经典”组成型表达同源物之间的进化关系问题。有证据表明，“非经典凝集素”代表了主要的进化线，并且它们的一些相应基因被用作编码存储蛋白样“经典”同源物的基因的模板。
During the last few years compelling evidence has been presented for the occurrence of cytoplasmic/nuclear plant lectins that are not detectable in normal plants but are only induced upon application of well-defined stress conditions. Since both the regulation of the expression and the subcellular location indicate that these 'non-classical lectins' are good candidates to play a physiologically important role as mediators of specific protein-carbohydrate-interactions within the plant cell, a critical assessment is made of the impact of these findings on the development of novel concepts about the role of plant lectins. Based on an analysis of the biochemical, molecular and evolutionary data of a jasmonate-induced chitin-binding lectin from tobacco leaves and a salt/jasmonate-induced leaf lectin from rice it is concluded that these lectins most probably interact with endogenous glycans located within the cytoplasmic/nuclear compartment of the plant cell. Several working mechanisms are proposed to explain how these inducible lectins may fulfill an important regulatory or structural role in stressed cells. In addition, the question of the evolutionary relationship(s) between the newly discovered inducible lectins and their 'classical' constitutively expressed homologs is addressed. Evidence is presented that the 'non-classical lectins' represent the main evolutionary line and that some of their corresponding genes were used as templates for genes encoding storage protein-like 'classical' homologs.