A recently evolved hexose transporter variant confers resistance to multiple pathogens in wheat
A recently evolved hexose transporter variant confers resistance to multiple pathogens in wheat
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DOI：
10.1038/ng.3439
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发表时间：
2015-12-01
期刊：
影响因子：
30.8
通讯作者：
Lagudah, Evans
中科院分区：
文献类型：
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作者：
Moore, John W.;Herrera-Foessel, Sybil;Lagudah, Evans
由于有许多病原体物种引起疾病并限制作物产量，例如小麦（Triticum aestivum），因此提供对多种病原体的抗性的单个基因在作物改良中是有价值的（1，2）。多病原体抗性的机制基础在很大程度上是未知的。在这里，我们使用比较基因组学，诱变和转化分离小麦Lr67基因，赋予部分抗性所有三个小麦锈病病原体物种和白粉病。Lr67抗性基因编码预测的己糖转运蛋白（LR67res），其与相同蛋白质的易感形式（LR67sus）的不同之处在于在正己糖转运蛋白中保守的两个氨基酸。糖摄取试验表明，LR 67 sus，和相关蛋白编码的同源等位基因，功能作为高亲和力的葡萄糖转运蛋白。LR67 res通过与这些功能性转运蛋白的异源二聚化发挥显性负效应以减少葡萄糖摄取。受感染叶片中己糖运输的改变可以解释其减少多种活体营养病原体物种生长的能力。
As there are numerous pathogen species that cause disease and limit yields of crops, such as wheat (Triticum aestivum), single genes that provide resistance to multiple pathogens are valuable in crop improvement(1,2). The mechanistic basis of multi-pathogen resistance is largely unknown. Here we use comparative genomics, mutagenesis and transformation to isolate the wheat Lr67 gene, which confers partial resistance to all three wheat rust pathogen species and powdery mildew. The Lr67 resistance gene encodes a predicted hexose transporter (LR67res) that differs from the susceptible form of the same protein (LR67sus) by two amino acids that are conserved in orthologous hexose transporters. Sugar uptake assays show that LR67sus, and related proteins encoded by homeoalleles, function as high-affinity glucose transporters. LR67res exerts a dominant-negative effect through heterodimerization with these functional transporters to reduce glucose uptake. Alterations in hexose transport in infected leaves may explain its ability to reduce the growth of multiple biotrophic pathogen species.