Desertihabitans aurantiacus gen. nov., sp. nov., a novel member of the family Propionibacteriaceae
Desertihabitans aurantiacus gen. nov., sp. nov., a novel member of the family Propionibacteriaceae
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橙色荒漠菌属
DOI：
10.1099/ijsem.0.003519
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发表时间：
2019
影响因子：
2.8
通讯作者：
Zhang Yu-Qin
中科院分区：
文献类型：
--
作者：
Sun Ye;Wang Hao;Zhang Tao;Liu Wei-Hong;Liu Hong-Yu;Yu Li-Yan;Zhang Yu-Qin
采用多相分类法对从巴丹吉林沙漠土壤中分离到的一株放线菌CPCC 204711 T进行了分类地位的确定。分离的细胞为革兰氏染色阳性、需氧、非运动性球菌。在28 °C（范围20-40 °C）、pH 7.0（范围pH 6.0-8.0）和0- 1%NaCl浓度（范围0- 5%，w/v）下观察到良好的生长。 在全细胞水解物中检测到半乳糖、阿拉伯糖和核糖作为糖组分。肽聚糖类型为A3 γ（II-Dpm-Gly）。MK-9（H4）被检测为主要的甲基萘醌，二磷脂酰甘油、磷脂酰甘油、磷脂酰胆碱、几种未鉴别的糖脂和一种未鉴别的氨基糖脂被检测为主要极性脂质。主要脂肪酸是anteiso-C15：0。 基因组DNA G+C含量为73.1mol%。系统发育分析表明，菌株CPCC 204711 T属于丙酸杆菌科，与Mariniluteicoccus endophyticus YIM 2617 T的16 S rRNA基因序列相似性最高，为96.0%。 系统发育分析和表型特征均支持菌株CPCC 204711 T为丙酸杆菌科一新属的新种，命名为橙黄拟杆菌（propionibacteriaceae）。十一月，nov.，模式菌株为CPCC 204711 T（=KCTC 39977 T =DSM 105431 T）。
The taxonomic position of an actinobacterium isolated from a desert soil sample collected from Badain Jaran Desert, designated as CPCC 204711T, was established using a polyphasic approach. Cells of the isolate were Gram-staining-positive, aerobic, non-motile cocci. Good growth was observed at 28 °C (range 20–40 °C), pH 7.0 (range pH 6.0–8.0) and 0–1 % NaCl concentration (range 0–5 %, w/v). Galactose, arabinose and ribose were detected as the sugar compositions in the whole cell hydrolysates. The peptidoglycan type was A3gamma (ll-Dpm-Gly). MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids. The predominant fatty acid wasanteiso-C15 : 0. The genomic DNA G+C content was 73.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204711Taffiliated to the familyPropionibacteriaceae, in which the strain formed a distinct phylogenetic lineage next to the genusMariniluteicoccus ,with the highest 16S rRNA gene sequence similarity of 96.0 % toMariniluteicoccus endophyticusYIM 2617T. Both phylogenetic analysis and phenotypic characteristics supported that strain CPCC 204711Trepresents a novel species of a new genus in the familyPropionibacteriaceae, for which the nameDesertihabitans aurantiacusgen. nov., sp. nov. is proposed, with CPCC 204711T(=KCTC 39977T=DSM 105431T) as the type strain.