Selective colonization of insoluble substrates by human faecal bacteria
Selective colonization of insoluble substrates by human faecal bacteria
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DOI：
10.1111/j.1462-2920.2006.01186.x
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发表时间：
2007-03-01
影响因子：
5.1
通讯作者：
Flint, Harry J.
中科院分区：
文献类型：
--
作者：
Leitch, E. Carol McWilliam;Walker, Alan W.;Flint, Harry J.
不溶性植物多糖和内源性粘蛋白是人体结肠微生物的重要能量来源。这项研究的目的是确定特定的群落是否在这些底物上定居。以4个个体的粪便作为厌氧体外连续流系统的接种物，检测了小麦麸皮、高直链淀粉和猪胃粘蛋白的定植情况。回收的底物被广泛洗涤，其余紧密结合的细菌群落通过聚合酶链式反应-扩增16S rRNA基因序列和荧光原位杂交进行鉴定。底物对检测到的附着细菌种类有较大影响。从麸皮中检索到的序列以梭状芽胞杆菌聚集性XIVa细菌为主，包括未培养的哈氏梭状芽孢杆菌、直肠杆菌和玫瑰草属的近亲。还检测到类杆菌属。从淀粉中回收的最丰富的序列与培养种布鲁氏瘤胃球菌、青春型双歧杆菌、短双歧杆菌和E.rectale有关。从粘蛋白中回收的最常见的序列是来自双歧双歧杆菌和与乳酸瘤胃球菌相关的未培养细菌。这项研究表明，特定的细菌亚群很可能是特定不溶性结肠底物的主要定殖者。然而，对于给定的底物，寄主个体之间的主要定殖种可能不同。
Insoluble plant polysaccharides and endogenous mucin are important energy sources for human colonic microorganisms. The object of this study was to determine whether or not specific communities colonize these substrates. Using faecal samples from four individuals as inocula for an anaerobic in vitro continuous flow system, the colonization of wheat bran, high amylose starch and porcine gastric mucin was examined. Recovered substrates were extensively washed and the remaining tightly attached bacterial communities were identified using polymerase chain reaction-amplified 16S rRNA gene sequences and fluorescent in situ hybridization. The substrate had a major influence on the species of attached bacteria detected. Sequences retrieved from bran were dominated by clostridial cluster XIVa bacteria, including uncultured relatives of Clostridium hathewayi, Eubacterium rectale and Roseburia species. Bacteroides species were also detected. The most abundant sequences recovered from starch were related to the cultured species Ruminococcus bromii, Bifidobacterium adolescentis, Bifidobacterium breve and E. rectale. The most commonly recovered sequences from mucin were from Bifidobacterium bifidum and uncultured bacteria related to Ruminococcus lactaris. This study suggests that a specific subset of bacteria is likely to be the primary colonizers of particular insoluble colonic substrates. For a given substrate, however, the primary colonizing species may vary between host individuals.