Bacteriological study of autologous cryoprecipitate-derived fibrin glue as the operative sealant
Bacteriological study of autologous cryoprecipitate-derived fibrin glue as the operative sealant
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DOI：
10.1111/j.1365-3148.2005.00604.x
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发表时间：
2005-10-01
影响因子：
1.5
通讯作者：
Inagawa, H
中科院分区：
文献类型：
--
作者：
Kinoshita, Y;Udagawa, H;Inagawa, H
纤维蛋白胶(FG)在心血管外科或矫形外科等许多手术中经常用来密封和覆盖吻合口。然而，在胃肠道手术中，吻合口可能会受到污染，而FG可能会促进细菌生长，增加渗漏的风险。本研究的目的是检测冷沉淀物衍生FG(CryoFG)对细菌生长的影响。对细菌在CryoFG和商业FG(Beriplast PO)上的生长进行了评估，并与对照培养基上的细菌生长进行了比较。此外，通过加热灭活或在实验环境中添加豚鼠补体来评估补体活性。CryoFG对大肠杆菌的生长有抑制作用，而商品化的FG则无此作用。CryoFG的热灭活可抑制其杀菌作用。在热灭活的CryoFG中加入豚鼠补体可基本恢复杀菌活性，提示补体的重要作用。本研究表明，CryoFG保留了补体活性，抑制了大肠杆菌的体外生长。因此，我们认为CryoFG在胃肠道手术吻合中的应用不仅安全，而且具有减少细菌感染的优点。
Fibrin glue (FG) is frequently used to seal and cover the anastomoses in many operations such as cardiovascular surgery or orthopaedic surgery. However, in case of gastrointestinal surgery, anastomoses are potentially contaminated, and FG may promote bacterial growth, increasing the risk of leakage. The purpose of this study was to examine the effect of cryoprecipitate-derived FG (CryoFG) on bacterial growth. Bacterial growth on the CryoFG and on the commercial FG (Beriplast PO) was evaluated and compared with that on control medium. In addition, the complement activities were evaluated by heat inactivation or addition of guinea-pig complement to the experimental settings. The CryoFG inhibited the growth of Escherichia coli, whereas the commercial FG had no effect. Heat inactivation of the CryoFG inhibited the bactericidal effect of CryoFG. Addition of guinea-pig complement to the heat-inactivated CryoFG could almost restore the bactericidal activity, suggesting the important role of complement. This study showed that the CryoFG preserved the complement activity, which inhibited the in vitro growth of E. coli. Therefore, we concluded that the application of the CryoFG for gastrointestinal surgical anastomoses not only would be safe but also has the advantage of reducing bacterial infection.