Functional connectome of the striatal medium spiny neuron.
Functional connectome of the striatal medium spiny neuron.
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纹状体培养基神经元的功能连接组。
DOI：
10.1523/jneurosci.3833-10.2011
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发表时间：
2011-01-26
期刊：
影响因子：
--
通讯作者：
Rayport S
中科院分区：
文献类型：
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作者：
Chuhma N;Tanaka KF;Hen R;Rayport S
从运动障碍到成瘾和精神分裂症的多巴胺系统障碍涉及纹状体中棘神经元，但它们的功能连接一直难以全面确定。我们产生了一个小鼠的条件性视紫红质-2表达仅限于中型多刺神经元和评估的特异性和强度的内在连接在纹状体，他们的预测苍白球，和黑质。在纹状体中，中型多刺神经元与其他MSN和紧张性活性胆碱能中间神经元连接，但不与快速尖峰GABA中间神经元连接。在苍白球中，中等棘状神经元与一类电生理识别的神经元连接较强，但与另一类神经元连接较弱。在黑质，中等棘状神经元与GABA连接，但不与多巴胺神经元连接。投射到苍白球表现出完全D2介导的突触前抑制，而投射到黑质表现出D1介导的突触前促进。这种光遗传学方法定义了纹状体中棘神经元的功能性连接体。
Dopamine system disorders ranging from movement disorders to addiction and schizophrenia involve striatal medium-spiny neurons, yet their functional connectivity has been difficult to determine comprehensively. We generated a mouse with conditional Channelrhodopsin-2 expression restricted to medium-spiny neurons and assessed the specificity and strength of their intrinsic connections in the striatum, their projections to the globus pallidus, and to the substantia nigra. In the striatum, medium-spiny neurons connected with other MSNs and tonically-active cholinergic interneurons, but not with fast-spiking GABA interneurons. In the globus pallidus, medium-spiny neurons connected strongly with one class of electrophysiologically identified neurons, but weakly with the other. In the substantia nigra, medium-spiny neurons connected strongly with GABA, but not with dopamine neurons. Projections to the globus pallidus showed solely D2-mediated presynaptic inhibition, while projections to the substantia nigra showed D1-mediated presynaptic facilitation. This optogenetic approach defines the functional connectome of the striatal medium-spiny neuron.