Dinickel-Salphen Complexes as Binders of Human Telomeric Dimeric G-Quadruplexes
Dinickel-Salphen Complexes as Binders of Human Telomeric Dimeric G-Quadruplexes
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Dinickel-Salphen 复合物作为人端粒二聚体 G-四链体的结合剂
DOI：
10.1002/chem.201700276
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发表时间：
2017-04-03
影响因子：
4.3
通讯作者：
Vilar, Ramon
中科院分区：
文献类型：
--
作者：
Zhou, Chun-Qiong;Liao, Ting-Cong;Vilar, Ramon
合成了三个新的聚醚型二镍-水杨酸配合物（2a-c），并通过核磁共振谱、质谱和元素分析对其进行了表征。用紫外/维斯滴定法、荧光光谱法、圆二色谱法和电泳法测定了这些配合物和母体单镍配合物（1）对二聚四链体DNA的结合亲和力和选择性。这些研究表明，具有最长聚醚接头的二镍-水杨酸络合物（2c）比具有较短聚醚接头的二镍-水杨酸络合物（2a和2b）对二聚四链体（相对于单体四链体）具有更高的结合亲和力和选择性。复合物2c对具有一个TTA接头的人端粒二聚体四链体的选择性也高于单核苷酸复合物1。基于光谱数据，提出了配合物2c与所研究的二聚体G-四链体DNA之间可能的结合模式。
Three new polyether-tethered dinickel-salphen complexes (2a-c) have been synthesized and fully characterized by NMR spectroscopy, mass spectrometry, and elemental analyses. The binding affinity and selectivity of these complexes and of the parent mono-nickel complex (1) towards dimeric quadruplex DNA have been determined by UV/Vis titrations, fluorescence spectroscopy, CD spectroscopy, and electrophoresis. These studies have shown that the dinickel-salphen complex with the longest polyether linker (2c) has higher binding affinity and selectivity towards dimeric quadruplexes (over monomeric quadruplexes) than the dinickel-salphen complexes with the shorter polyether linkers (2a and 2b). Complex 2c also has higher selectivity towards human telomeric dimeric quadruplexes with one TTA linker than the monometallic complex 1. Based on the spectroscopic data, a possible binding mode between complex 2c and the dimeric G-quadruplex DNA under study is proposed.