High-resolution mapping of two rice brown planthopper resistance genes, Bph20(t) and Bph21(t), originating from Oryza minuta
High-resolution mapping of two rice brown planthopper resistance genes, Bph20(t) and Bph21(t), originating from Oryza minuta
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DOI：
10.1007/s00122-009-1125-z
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发表时间：
2009-11-01
影响因子：
5.4
通讯作者：
Koh, Hee-Jong
中科院分区：
文献类型：
--
作者：
Rahman, Md Lutfor;Jiang, Wenzhu;Koh, Hee-Jong
褐飞虱（BPH）是水稻最具破坏性的害虫之一。野生水稻品种是开发抗性品种的抗性基因的宝贵来源。使用衍生自 Oryza minuta（登录号 101141）的基因渗入系“IR71033-121-15”和易受影响的韩国粳稻品种“Junambyeo”杂交的 F-2 群体，对 BPH 抗性进行了基于分子标记的遗传分析。使用 190 个 F-3 家族评估了对 BPH（生物型 1）的抗性。鉴定出两个主要数量性状基因座（QTL）和标记区间之间两个显着的双基因上位相互作用。一个QTL定位于位于4号染色体短臂的193.4-kb区域，另一个QTL定位于位于12号染色体长臂的194.0-kb区域。这两个QTL相加增强了对BPH的抗性。在每个基因座上开发了与两个抗性 QTL 共分离的标记。将这两个QTL的物理图位位置与之前报道的BPH抗性基因进行比较，我们得出结论，这些主要QTL是新的BPH抗性位点，并将它们命名为4号染色体上的Bph20(t)和12号染色体上的Bph21(t)。这是野生物种O. minuta的BPH抗性基因的首次报道。这里报道的这两个新基因和标记将有助于对褐飞虱抗性新来源感兴趣的水稻育种计划。
Brown planthopper (BPH) is one of the most destructive insect pests of rice. Wild species of rice are a valuable source of resistance genes for developing resistant cultivars. A molecular marker-based genetic analysis of BPH resistance was conducted using an F-2 population derived from a cross between an introgression line, 'IR71033-121-15', from Oryza minuta (Accession number 101141) and a susceptible Korean japonica variety, 'Junambyeo'. Resistance to BPH (biotype 1) was evaluated using 190 F-3 families. Two major quantitative trait loci (QTLs) and two significant digenic epistatic interactions between marker intervals were identified for BPH resistance. One QTL was mapped to 193.4-kb region located on the short arm of chromosome 4, and the other QTL was mapped to a 194.0-kb region on the long arm of chromosome 12. The two QTLs additively increased the resistance to BPH. Markers co-segregating with the two resistance QTLs were developed at each locus. Comparing the physical map positions of the two QTLs with previously reported BPH resistance genes, we conclude that these major QTLs are new BPH resistance loci and have designated them as Bph20(t) on chromosome 4 and Bph21(t) on chromosome 12. This is the first report of BPH resistance genes from the wild species O. minuta. These two new genes and markers reported here will be useful to rice breeding programs interested in new sources of BPH resistance.