Silencing arginine kinase/integrin β1 subunit by transgenic plant expressing dsRNA inhibits the development and survival of Plutella xylostella
Silencing arginine kinase/integrin β1 subunit by transgenic plant expressing dsRNA inhibits the development and survival of Plutella xylostella
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DOI：
10.1002/ps.5701
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发表时间：
2020-05-01
影响因子：
4.1
通讯作者：
Yang, Guang
中科院分区：
文献类型：
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作者：
Fu, Shu;Liu, Zhaoxia;Yang, Guang
背景小菜蛾是一种危害包括农作物在内的十字花科植物的农业害虫。植物介导的RNA干扰(RNAi)目前正被用于植物保护。在本研究中，我们研究了针对小菜蛾精氨酸激酶(PxAK)和整合素β(1)亚单位(Pxβ)基因的双链RNA(DsRNA)在拟南芥转基因植株中的暴露反应。结果获得并验证了PxAK基因384个碱基片段(DsAK)、Pxβ基因497个碱基片段(ds beta植株)以及两种基因组合的881bp片段(dsAK-beta植株)的转基因植株。用这些转基因植株进行昆虫生物测定表明，影响了小菜蛾的发育，导致发育时间延长，幼虫体重和化蛹率降低。小菜蛾在dsAK植物、ds beta植物和dsAK-beta植物上的死亡率分别为25.0%、22.5%和30.0%，均高于野生型植物的7.5%。在转录水平上，转基因植株对小菜蛾体内PxAK和Pxβ的抑制率为26.6%~79.7%。结论植物介导的针对单基因或同时针对PxAK和Pxβ的RNAi可能具有控制小菜蛾的潜力。(三)2019年化学工业学会
Background Plutella xylostella is a devastating agricultural insect pest of cruciferous plants, including crops. Plant-mediated RNA interference (RNAi) is currently being developed for plant protection. In this study, we investigated the response of P. xylostella exposed to transgenic Arabidopsis thaliana plants that expressed double-stranded RNA (dsRNA) targeting P. xylostella genes of arginine kinase (PxAK) and integrin beta(1) subunit (Px beta).RESULTS Transgenic plants producing dsRNAs of the 384-bp fragment of PxAK (dsAK plants), the 497-bp fragment of Px beta (ds beta plants), and the 881 bp of the combination of both genes (dsAK-beta plants) were generated and verified. Insect bioassay with these transgenic plants showed that the development of P. xylostella was affected, causing longer developmental time, and lower pupal weight and pupation rate. P. xylostella mortality rates were 25.0% when exposed to dsAK plants, 22.5% with ds beta plants, and 30.0% with dsAK-beta plants, which were all higher than 7.5% for the wild-type plant. PxAK and Px beta in P. xylostella were suppressed by 26.6-79.7% at the transcription level by the transgenic plants.CONCLUSION These results suggest that plant-mediated RNAi targeting single gene or both PxAK and Px beta may have the potential to control P. xylostella. (c) 2019 Society of Chemical Industry