Decreased human immunodeficiency virus type 1 plasma viremia during antiretroviral therapy reflects downregulation of viral replication in lymphoid tissue.
Decreased human immunodeficiency virus type 1 plasma viremia during antiretroviral therapy reflects downregulation of viral replication in lymphoid tissue.
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抗逆转录病毒治疗期间人类免疫缺陷病毒 1 型血浆病毒血症的减少反映了淋巴组织中病毒复制的下调。
DOI：
10.1073/pnas.92.13.6017
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发表时间：
1995
影响因子：
11.1
通讯作者：
Steigbigel,RT
中科院分区：
文献类型：
--
作者：
Cohen,OJ;Pantaleo,G;Holodniy,M;Schnittman,S;Niu,M;Graziosi,C;Pavlakis,GN;Lalezari,J;Bartlett,JA;Steigbigel,RT
尽管在外周血中测量的几种免疫学和病毒学标记物可用于预测人类免疫缺陷病毒（HIV）疾病的加速进展，但它们评估抗逆转录病毒治疗反应的有效性以及准确反映淋巴器官变化的能力仍不清楚。在本研究中，分析了抗逆转录病毒治疗期间外周血和淋巴组织中某些病毒学标志物的变化。 16 名接受齐多夫定抗逆转录病毒治疗≥6 个月的 HIV 感染者被随机分配继续单独使用齐多夫定或加用去羟肌苷 8 周。在基线时和 8 周后进行淋巴结活检。通过半定量聚合酶链反应测定测定从外周血和淋巴结分离的单核细胞中的病毒负荷（即每 10(6) 个单核细胞的 HIV DNA 拷贝数）和病毒复制以及血浆病毒血症。继续单独使用齐多夫定的患者外周血和淋巴结中的病毒学和免疫学标志物保持不变。相比之下，在治疗方案中添加去羟肌苷的六名患者中，有四名观察到淋巴结中病毒复制的减少，这与血浆病毒血症的减少有关。这些结果表明，抗逆转录病毒治疗期间检测到的血浆病毒血症的减少反映了淋巴组织中病毒复制的下调。
Although several immunologic and virologic markers measured in peripheral blood are useful for predicting accelerated progression of human immunodeficiency virus (HIV) disease, their validity for evaluating the response to antiretroviral therapy and their ability to accurately reflect changes in lymphoid organs remain unclear. In the present study, changes in certain virologic markers have been analyzed in peripheral blood and lymphoid tissue during antiretroviral therapy. Sixteen HIV-infected individuals who were receiving antiretroviral therapy with zidovudine for > or = 6 months were randomly assigned either to continue on zidovudine alone or to add didanosine for 8 weeks. Lymph node biopsies were performed at baseline and after 8 weeks. Viral burden (i.e., HIV DNA copies per 10(6) mononuclear cells) and virus replication in mononuclear cells isolated from peripheral blood and lymph node and plasma viremia were determined by semiquantitative polymerase chain reaction assays. Virologic and immunologic markers remained unchanged in peripheral blood and lymph node of patients who continued on zidovudine alone. In contrast, a decrease in virus replication in lymph nodes was observed in four of six patients who added didanosine to their regimen, and this was associated with a decrease in plasma viremia. These results indicate that decreases in plasma viremia detected during antiretroviral therapy reflect downregulation of virus replication in lymphoid tissue.