miR-290 contributes to the low abundance of cyclin D1 protein in mouse embryonic stem cells
miR-290 contributes to the low abundance of cyclin D1 protein in mouse embryonic stem cells
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DOI：
10.1093/abbs/gmx049
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发表时间：
2017-07-01
影响因子：
3.7
通讯作者：
Jin, Youxin
中科院分区：
文献类型：
--
作者：
Gong, Zizhen;Wang, Detao;Jin, Youxin
小鼠miR-290簇miRNA在早期胚胎和胚胎生殖细胞中特异性表达。这些miRNAs在维持多能性和自我更新中起着关键作用。在这里，我们发现Cyclin D1是miR-290簇miRNA的直接靶基因。在多能和非多能细胞以及分化中的CGR 8细胞中，观察到Cyclin D1蛋白和miR-290簇miRNA的表达之间的负相关性。抑制miR-290簇miRNAs可使CGR 8小鼠干细胞阻滞于G1期并减缓细胞增殖。由于miR-290簇miRNAs是最主要的干细胞特异性miRNAs，我们的研究结果揭示了小鼠胚胎干细胞中Cyclin D1缺失的重要原因。
Mouse miR-290 cluster miRNAs are expressed specifically in early embryos and embryonic germ cells. These miRNAs play critical roles in the maintenance of pluripotency and self-renewal. Here, we showed that Cyclin D1 is a direct target gene of miR-290 cluster miRNAs. Negative relationships between the expression of Cyclin D1 protein and miR-290 cluster miRNAs in pluripotent and non-pluripotent cells, as well as in differentiating CGR8 cells were observed. Inhibition of miR-290 cluster miRNAs could arrest cells at the G1 phase and slow down the cell proliferation in CGR8 mouse stem cells. Since miR-290 cluster miRNAs are the most dominant stem-cell-specific miRNAs, our results revealed an important cause for the absence of Cyclin D1 in mouse embryonic stem cells.