The Cpx Envelope Stress Response
The Cpx Envelope Stress Response
复制标题
Cpx 包络应激反应
DOI：
10.1128/9781555815806.ch5
复制
发表时间：
2007
影响因子：
20.1
通讯作者：
T. Raivio
中科院分区：
文献类型：
--
作者：
T. Raivio
本章总结了 Cpx 包膜应激反应的识别和表征的研究，并重点介绍了暗示各种 Cpx 影响的细胞表型的最新工作。 Silverman 和同事为我们目前了解 Cpx 包络应力反应提供了核心。 Cpx 包络应力反应由典型的两部分调节系统调节。除了作为激活子和阻遏子的功能外，最近还提出了 CpxR~P 在转录中的一个新作用，即增效子。研究人员通过对生物膜和浮游细胞群进行微阵列分析表明，Cpx 调节基因 cpxP 和间谍在生物膜中高度上调，并且 cpxP 和 cpxR 突变体形成的生物膜质量和底物覆盖率降低。研究表明，嗜肺军团菌和小肠结肠炎耶尔森氏菌中的 CpxR 同源物可能参与分别编码 IV 型分泌系统和 degP 的 icm-dot 基因的转录，这两者都是毒力所必需的。对野生型和 Cpx 诱导菌株中 TraJ、TraY 和 TraM 蛋白半衰期的分析表明，当 Cpx 反应被激活时，TraJ 稳定性会明显降低。这项研究首次表明，除了包膜中发现的蛋白外，Cpx 反应还可能影响细胞内蛋白的命运。 Cpx 包络应激反应与其他适应性反应表现出显着的连通性。尽管 CpxA 能够感知错误折叠的包膜蛋白并被其激活已成为教条，但所涉及的机制仍然难以捉摸。
This chapter summarizes studies that led to identification and characterization of the Cpx envelope stress response and highlights recent work that hints at a diverse range of Cpx-influenced cellular phenotypes. Silverman and colleagues provided the kernels for our current understanding of the Cpx envelope stress response. The Cpx envelope stress response is regulated by a typical two-component regulatory system. In addition to functioning as an activator and a repressor, a new role for CpxR~P in transcription has recently been put forth, that of potentiator. Researchers have shown by microarray analysis of biofilm and planktonic populations of cells that the Cpx-regulated genes cpxP and spy were highly up-regulated in biofilms and that cpxP and cpxR mutants formed biofilms with reduced mass and substrate coverage. It has been shown that CpxR homologues in Legionella pneumophila and Yersinia enterocolitica are likely involved in transcription of the icm-dot genes encoding the type IV secretion system and degP, respectively, both of which are necessary for virulence. Analysis of TraJ, TraY, and TraM protein half-lives in wild-type and Cpx-induced strains indicated that TraJ stability was specifically decreased when the Cpx response was activated. This study was the first to indicate that the Cpx response might influence the fates of intracellular proteins, in addition to those found in the envelope. The Cpx envelope stress response exhibits significant connectivity to other adaptive responses. Although it has become dogma that CpxA senses and is activated by misfolded envelope proteins, the mechanism(s) involved remain elusive.
登录
查看更多内容
影响因子：
10.5
作者：
ERICKSON, JW;GROSS, CA
通讯作者：
GROSS, CA
DOI：
10.1073/pnas.0505818102
发表时间：
2005-11
影响因子：
11.1
作者：
Jyl S Matson;V. DiRita
通讯作者：
Jyl S Matson;V. DiRita
DOI：
10.1073/pnas.0508936102
发表时间：
2005-12-06
影响因子：
11.1
作者：
Isaac, DD;Pinkner, JS;Silhavy, TJ
通讯作者：
Silhavy, TJ
影响因子：
10.5
作者：
DANESE, PN;SNYDER, WB;SILHAVY, TJ
通讯作者：
SILHAVY, TJ
大肠杆菌 K12 的 Cpx 蛋白。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Albin,R;Weber,R;Silverman,PM
通讯作者：
Silverman,PM