Erythroid colony formation in cultures of human marrow: Effect of leukocyte conditioned medium
Erythroid colony formation in cultures of human marrow: Effect of leukocyte conditioned medium
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人骨髓培养物中红细胞集落的形成：白细胞条件培养基的影响
DOI：
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发表时间：
1977
影响因子：
5.6
通讯作者：
M. Aye
中科院分区：
文献类型：
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作者：
M. Aye
基于细胞粘附到聚酯纤维柱上的不同能力，描述了一种将人骨髓细胞分离成非粘附部分和粘附部分的方法。研究了这种分离方法对红系祖细胞集落形成的影响。与未分离的群体相比，研究发现，在培养物中加入白细胞条件培养基后，非贴壁细胞的红细胞生成素依赖性红细胞集落形成可以显著增强。从人胚胎肾培养上清液中获得的粒细胞集落刺激活性部分纯化制剂也检测到类似的红细胞增强活性。
A method, based on the differing capacities of cells to adhere to a column of polyester fibres, has been described for separating human bone marrow cells into a nonadherent and an adherent fraction. The effect of this cell separation procedure on colony formation by erythroid progenitor cells was investigated. In contrast to the unseparated population, it was found that erythropoietin‐dependent erythroid colony formation by nonadherent cells could be considerably enhanced by the addition of leukocyte conditioned medium to the cultures. Similar erythroid enhancing activity was also detected in a partially purified preparation of granulocytic colony stimulating activity obtained from human embryo kidney culture supernatants.