Diversity of Gram negative bacteria antagonistic against major pathogens of rice from rice seed in the tropic environment
Diversity of Gram negative bacteria antagonistic against major pathogens of rice from rice seed in the tropic environment
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DOI：
10.1631/jzus.2003.0463
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发表时间：
2003-07
影响因子：
3.2
通讯作者：
G. Xie;A. Soad;J. Swings;T. Mew
中科院分区：
文献类型：
--
作者：
G. Xie;A. Soad;J. Swings;T. Mew
With the use of a seed washing technique, more than 4000 Gram negative bacteria were isolated by two improved isolation methods from 446 batches of 1 kg rice seed samples obtained from 22 provinces in the Philippines. They were initially characterized on the basis of colony morphology and results of biochemical and pathogenicity tests. Six hundred and fifty-two strains were further identified by Biolog, from which 133 were selected for fatty acid methyl ester (FAME) analysis together with 80 standard reference! strains. Six-teen species or types ofPseudomonas and 17 genera of non-pseudomonads were identified, more than one third of which have not been recorded in rice. The most predominant species observed wereP. putida andP. fulva. About 17% of the strains ofPseudomonas and 2% of the non-pseudomonads were antagonistic to one or more fungal or bacterial pathogens of rice. Rice seed is an important source of biological control agents.
