Distinct metabolic differences between various human cancer and primary cells
Distinct metabolic differences between various human cancer and primary cells
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DOI：
10.1002/elps.201300228
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发表时间：
2013-10-01
期刊：
影响因子：
2.9
通讯作者：
Gottfried, Eva
中科院分区：
文献类型：
--
作者：
Dettmer, Katja;Vogl, Franziska C.;Gottfried, Eva
近年来，人们对癌细胞代谢和分泌的癌代谢物在调节肿瘤间质中的作用重新产生了兴趣。使用非靶向和靶向LC和GC-MS方法的组合，三种白血病、两种黑色素瘤、三种肾细胞癌、两种结肠直肠腺癌、四种肝细胞癌、三种乳腺癌、两种膀胱癌和一种胶质母细胞瘤细胞系的外泌代谢体，以及人黑素细胞、肝细胞、单核细胞、CD 4和CD 8淋巴细胞的五种原代培养物，在相同的条件下培养，进行了研究。代谢足迹的无监督亲和传播聚类产生了五个不同的聚类，主要根据组织来源对所研究的细胞培养物进行分组。所有肿瘤细胞的共同预期特征是高乳酸盐产生。细胞外精氨酸和烟酰胺是正常和肿瘤肝细胞之间的主要判别指标。此外，还观察到二肽和三肽同化的显著差异。这一发现似乎强调了肽在满足许多癌症增加的生物能量和生物合成需求方面的重要性。
Recent years have seen resurging interest in cancer cell metabolism and the role of secreted cancer metabolites in modulating the tumor stroma. Using a combination of nontargeted and targeted LC and GC-MS methods, the exometabolomes of three leukemia, two melanoma, three renal cell carcinoma, two colorectal adenocarcinoma, four hepatocellular carcinoma, three breast cancer, two bladder carcinoma, and one glioblastoma cell line, as well as five primary cultures of human melanocytes, hepatocytes, monocytes, CD4 and CD8 lymphocytes, that had been all cultivated under identical conditions, were investigated. Unsupervised affinity propagation clustering of the metabolic footprints yielded five distinct clusters that grouped the investigated cell cultures mainly according to the tissue of origin. A common expected feature of all neoplastic cells was high lactate production. Extracellular arginine and nicotinamide were major discriminants between normal and neoplastic hepatocytes. Further, significant differences in the assimilation of di- and tripeptides were observed. This finding appears to underscore the importance of peptides for meeting the increased bioenergetic and biosynthetic demands of many cancers.