Vimentin in the central nervous system. A study of the mesenchymal-type intermediate filament-protein in Wallerian degeneration and in postnatal rat development by two-dimensional gel electrophoresis.
Vimentin in the central nervous system. A study of the mesenchymal-type intermediate filament-protein in Wallerian degeneration and in postnatal rat development by two-dimensional gel electrophoresis.
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DOI：
10.1111/j.1432-0436.1982.tb01248.x
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发表时间：
1982-09
期刊：
影响因子：
--
通讯作者：
D. Dahl;P. Strocchi;A. Bignami
中科院分区：
文献类型：
--
作者：
D. Dahl;P. Strocchi;A. Bignami
通过双向凝胶电泳鉴定了大鼠沃勒变性视神经的尿素提取物和大鼠脑和脊髓在出生后发育过程中的细胞骨架制剂中的中间丝蛋白。神经胶质细胞酸性蛋白（GFA）和波形蛋白是沃勒变性后的主要视神经蛋白。波形蛋白（Vimentin）是新生儿中枢神经系统（CNS）的主要细胞骨架成分，然后逐渐减少，直到在成熟的脑和脊髓中几乎不可识别。波形蛋白的减少与GFA蛋白的增加同时发生。在脑和脊髓的细胞骨架制剂以及正常视神经的尿素提取物中鉴定出表观分子量为61，000、等电点为5.6的蛋白质。在变性视神经中，该蛋白与形成神经丝三联体的多肽一起消失。
Intermediate filament proteins were identified by two-dimensional gel electrophoresis in urea extracts of rat optic nerves undergoing Wallerian degeneration and in cytoskeletal preparations of rat brain and spinal cord during postnatal development. The glial fibrillary acidic (GFA) protein and vimentin were the major optic nerve proteins following Wallerian degeneration. Vimentin was a major cytoskeletal component of newborn central nervous system (CNS) and then progressively decreased until it became barely identifiable in mature brain and spinal cord. The decrease of vimentin occurred concomitantly with an increase in GFA protein. A protein with the apparent molecular weight of 61,000 and isoelectric point of 5.6 was identified in both cytoskeletal preparations of brain and spinal cord, and in urea extracts of normal optic nerves. The protein disappeared together with the polypeptides forming the neurofilament triplet in degenerated optic nerves.