Effect of chlorhexidine and benzalkonium chloride on bacterial biofilm formation
Effect of chlorhexidine and benzalkonium chloride on bacterial biofilm formation
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DOI：
10.1111/j.1472-765x.2007.02249.x
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发表时间：
2007-12-01
影响因子：
2.4
通讯作者：
Di Martino, P.
中科院分区：
文献类型：
--
作者：
Houari, A.;Di Martino, P.
为了研究防腐剂对细菌生物被膜形成的影响，在微量平板上检测了防腐剂对细菌生物被膜形成和浮游生长的影响。对大肠杆菌G1473和氯己定、肺炎克雷伯氏菌CF504和铜绿假单胞菌PAO1在氯己定和苯扎氯铵存在下，生物膜的发育和浮游生长都有影响。对于PAO1(洗必泰存在时)和CF504(苯扎氯铵存在时)，防腐剂浓度比生物膜形成低四倍时，浮游生长受到显著抑制。对于表皮葡萄球菌CIP53124，在最小抑菌浓度(MIC)的存在下，生物被膜的形成完全被抑制。为了Staph。1/2、1/4和1/8MIC的洗必泰和1/8、1/16和1/32MIC的苯扎溴铵对表皮的生物被膜形成有明显的促进作用，但对浮游细菌的生长无明显影响。亚致死性阳离子防腐剂暴露可能通过生物被膜诱导葡萄球菌的持久性。
To study the effect of antiseptics on bacterial biofilm formation.Biofilm formation and planktonic growth were tested in microtiter plates in the presence of antiseptics. For Escherichia coli G1473 in the presence of chlorhexidine or benzalkonium chloride, for Klebsiella pneumoniae CF504 in the presence of chlorhexidine and for Pseudomonas aeruginosa PAO1 in the presence of benzalkonium chloride, biofilm development and planktonic growth were affected at the same concentrations of antiseptics. For PAO1 in the presence of chlorhexidine and CF504 in the presence of benzalkonium chloride, planktonic growth was significantly inhibited by a fourfold lower antiseptic concentration than biofilm development. For Staphylococcus epidermidis CIP53124 in the presence of antiseptics at the minimal inhibitory concentration (MIC), a total inhibition of biofilm formation was observed. For Staph. epidermidis exposed to chlorhexidine at 1/2, 1/4 and 1/8 MIC, or to benzalkonium chloride at 1/8, 1/16 or 1/32 MIC, biofilm formation was increased from 11.4% to 22.5% without any significant effect onto planktonic growth.Chlorhexidine and benzalkonium chloride inhibited biofilm formation of different bacterial species but were able to induce biofilm development for the Staph. epidermidis CIP53124 strain at sub-MICs.Sublethal exposure to cationic antiseptics may contribute to the persistence of staphylococci through biofilm induction.